热解质谱法作为白色念珠菌菌株间鉴别的一种方法。

Pyrolysis mass spectrometry as a method for inter-strain discrimination of Candida albicans.

作者信息

Magee J T, Hindmarch J M, Duerden B I, Mackenzie D W

机构信息

Department of Microbiology, Children's Hospital, Sheffield, UK.

出版信息

J Gen Microbiol. 1988 Oct;134(10):2841-7. doi: 10.1099/00221287-134-10-2841.

DOI:10.1099/00221287-134-10-2841

PMID:3076175

Abstract

A claim that Candida albicans strains NCPF 3153 and B311 were identical was investigated. Authentic strains were shown to be distinct (P less than 0.1%) by pyrolysis mass spectrometry (PyMS). Of twelve strains, provided as clones of NCPF 3153, seven were authenticated, one yielded an equivocal result and four were distinct from both NCPF 3153 and B311. Of eight B311 clones, six were authenticated and two yielded equivocal results. Although five non-C. albicans yeast strains were identified as distinct from B311 and NCPF 3153, Torulopsis glabrata NCPF 3240 was identified as B311, and one clinical isolate of C. albicans as NCPF 3153. This could be explained by the specificity of the mathematical analysis for discrimination between the authentic strains.

摘要

对白色念珠菌菌株NCPF 3153和B311是否相同的说法进行了调查。通过热解质谱法（PyMS）显示，正宗菌株是不同的（P小于0.1%）。在作为NCPF 3153克隆提供的12个菌株中，7个被鉴定，1个结果不明确，4个与NCPF 3153和B311都不同。在8个B311克隆中，6个被鉴定，2个结果不明确。虽然鉴定出5个非白色念珠菌酵母菌株与B311和NCPF 3153不同，但光滑球拟酵母NCPF 3240被鉴定为B311，1株白色念珠菌临床分离株被鉴定为NCPF 3153。这可以通过用于区分正宗菌株的数学分析的特异性来解释。