Autophosphorylation of yeast hexokinase PII.

作者信息

Fernández R, Herrero P, Fernández E, Fernández T, López-Boado Y S, Moreno F

机构信息

Departamento de Biología Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.

出版信息

J Gen Microbiol. 1988 Sep;134(9):2493-8. doi: 10.1099/00221287-134-9-2493.

Autophosphorylation of hexokinase PII was studied using an enzyme purified from Saccharomyces cerevisiae. Incubation of this enzyme preparation with [gamma-32P]ATP and Mn2+ or Mg2+ gave a phosphoprotein of molecular mass 58,000 which corresponded to hexokinase PII. D-Xylose stimulated autophosphorylation of hexokinase PII. Dilution of hexokinase PII over a 10-fold concentration range did not change the specific activity of hexokinase PII autophosphorylation suggesting that it may occur by an intramolecular mechanism.