端粒重复序列结合因子1和TRF2在阿尔茨海默病中的表达及其与临床参数的相关性。

Expression of telomere repeat binding factor 1 and TRF2 in Alzheimer's disease and correlation with clinical parameters.

作者信息

Wu Qi, Han Dongyang, Zhang Jiangkuan, Li Xin

机构信息

a The Second Clinical Medical College , Zhengzhou University , Zhengzhou , China.

b Department of Neurology , The Second Affiliated Hospital of Zhengzhou University , Zhengzhou , China.

出版信息

Neurol Res. 2019 Jun;41(6):504-509. doi: 10.1080/01616412.2019.1580456. Epub 2019 Feb 14.

DOI:10.1080/01616412.2019.1580456

PMID:30761946

Abstract

OBJECTIVE

The objective of this study was to investigate the expression of serum telomere repeat binding factor 1 (TRF1) and TRF2 in patients with Alzheimer's disease (AD) and their correlation with clinicopathological features.

METHODS

Fifty AD subjects and 50 healthy controls were enrolled in this study. Enzyme-linked immunosorbent assay (ELISA) was used to determine the expression of TRF1 and TRF2 in the peripheral blood plasma. Correlation analysis was used to evaluate the correlation between the protein expression and AD clinical parameters.

RESULTS

The expression of TRF1 in peripheral blood serum of AD patients was significantly higher than that of the control group (t = 5.533, P < 0.01) and TRF2 was lower than the control group (t = 2.627, P = 0.010). The expression of TRF1 was positively correlated with the history of coronary heart disease (Spearman's r = 0.298，P = 0.035). The expression of TRF1 and TRF2 was positively correlated with age (Pearson's r1 = 0.830，P1 < 0.01；Pearson's r2 = 0.942，P2 < 0.01), BMI (Pearson's r1 = 0.791，P1 < 0.01；Pearson's r2 = 0.941，P2 < 0.01), Aβ42 (Pearson's r1 = 0.765，P1 < 0.01；Pearson's r2 = 0.926，P2 < 0.01) and Tau protein (Pearson's r1 = 0.648，P1 < 0.01；Pearson's r2 = 0.691，P2 < 0.01) in blood serum of AD patients. However, there was no significant difference between both proteins expression and the history of hypertension, diabetes or stroke.

CONCLUSION

TRF1 and TRF2 may be both specific to peripheral blood serum of AD, and may be related to the development of AD.

摘要

目的

本研究旨在探讨阿尔茨海默病（AD）患者血清端粒重复结合因子1（TRF1）和TRF2的表达及其与临床病理特征的相关性。

方法

本研究纳入了50例AD患者和50例健康对照者。采用酶联免疫吸附测定（ELISA）法检测外周血血浆中TRF1和TRF2的表达。采用相关性分析评估蛋白表达与AD临床参数之间的相关性。

结果

AD患者外周血血清中TRF1的表达显著高于对照组（t = 5.533，P < 0.01），TRF2低于对照组（t = 2.627，P = 0.010）。TRF1的表达与冠心病史呈正相关（Spearman相关系数r = 0.298，P = 0.035）。AD患者血清中TRF1和TRF2的表达与年龄（Pearson相关系数r1 = 0.830，P1 < 0.01；Pearson相关系数r2 = 0.942，P2 < 0.01）、体重指数（BMI）（Pearson相关系数r1 = 0.791，P1 < 0.01；Pearson相关系数r2 = 0.941，P2 < 0.01）、Aβ42（Pearson相关系数r1 = 0.765，P1 < 0.01；Pearson相关系数r2 = 0.926，P2 < 0.01）和Tau蛋白（Pearson相关系数r1 = 0.648，P1 < 0.01；Pearson相关系数r2 = 0.691，P2 < 0.01）呈正相关。然而，两种蛋白表达与高血压、糖尿病或中风病史之间均无显著差异。