Kandemir Nezahat, Çelik Ali, Ullah Fazal, Shah Syed Nasar, Zaman Wajid
Education Faculty, Department of Biology, Amasya University, Amasya, Turkey.
Art and Science Faculty, Department of Biology, Pamukkale University, Denizli, Turkey.
Microsc Res Tech. 2019 Jun;82(6):764-774. doi: 10.1002/jemt.23221. Epub 2019 Feb 14.
Iris L. is one of the important genus of family Iridaceae, consist of 56 taxa naturally occurred in Turkey. The similarities and variations in the subgenus overlapping the taxonomic positions of the species in the subgenera and needs anatomical assessment especially by microscopic techniques. In this study, the taxonomic significance of leaf anatomical characters of 10 Iris subgenus Scorpiris taxa were studied in detail and the relationship among these taxa were evaluated using microscopy techniques. Fresh leaf samples of species were fixed in 70% alcohol solution for anatomical observation under microscope. Eleven different micromorphological features were statistically analyzed to delimit the species in subgenus. Based on morphological and anatomical similarities, we studied relationships among; (1) ssp. turcica, ssp. caucasica, I. nezahatiae and I. pseudocaucasica; (2) correlation between ssp. turcica and ssp. caucasica; (3) association of I. galatica, I. persica, ssp. margaretiae and ssp. stenophylla with each other; (4) relationship between ssp. stenophylla and ssp. margaretiae; and (5) relevance between I. aucheri and I. peshmeniana. Moreover, the taxonomy of subgenus Scorpiris has been discussed in detail with novel and diagnostic features based on micromorphological physiognomies. We found that four species in this study are endemic to Turkey, while seven are critically endangered geophytes in the country. The leaf anatomical characteristics of 10 taxa were divided into three groups. Main aim of this research was to study the taxonomy of the complex subgenus Scorpiris through microscopic techniques.
鸢尾属是鸢尾科的重要属之一，在土耳其自然分布着56个分类群。亚属间的相似性和变异性与各亚属中物种的分类地位重叠，尤其需要通过显微镜技术进行解剖学评估。在本研究中，详细研究了10个鸢尾属蝎尾鸢尾亚属分类群叶片解剖特征的分类学意义，并使用显微镜技术评估了这些分类群之间的关系。将物种的新鲜叶片样本固定在70%酒精溶液中，以便在显微镜下进行解剖观察。对11种不同的微观形态特征进行了统计分析，以界定该亚属中的物种。基于形态和解剖学上的相似性，我们研究了以下几方面的关系：（1）土耳其鸢尾亚种、高加索鸢尾亚种、内扎哈蒂鸢尾和假高加索鸢尾；（2）土耳其鸢尾亚种和高加索鸢尾亚种之间的相关性；（3）加拉太鸢尾、波斯鸢尾、玛格丽特鸢尾亚种和狭叶鸢尾亚种之间的关联；（4）狭叶鸢尾亚种和玛格丽特鸢尾亚种之间的关系；（5）奥氏鸢尾和佩什梅尼鸢尾之间的相关性。此外，还基于微观形态特征，详细讨论了蝎尾鸢尾亚属的分类学，并提出了新的诊断特征。我们发现本研究中的4个物种为土耳其特有种，而该国还有7种处于极度濒危状态的地生植物。10个分类群的叶片解剖特征分为三组。本研究的主要目的是通过显微镜技术研究复杂的蝎尾鸢尾亚属的分类学。
