Laboratory of Environmental Medicine and Developmental Toxicology, Guangzhou and Guangdong Key Laboratory of Environmental Pollution and Health, School of Environment, Jinan University, Guangzhou 510632, Guangdong, China.
Laboratory of Environmental Medicine and Developmental Toxicology, Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515041, Guangdong, China; Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen 9713 GZ, the Netherlands.
Sci Total Environ. 2019 May 10;664:690-697. doi: 10.1016/j.scitotenv.2019.02.040. Epub 2019 Feb 5.
Lead (Pb) toxicity damages blood cells and disturbs the immune micro-environment. When Pb enters the circulatory system, >95% of Pb accumulates in erythrocytes. We therefore conducted this study to explore the long-term effect of Pb exposure on expression of erythrocyte adhesion molecules (CD44 and CD58) and related downstream cytokine concentrations. We enrolled a total of 267 preschool children, 2-7 years of age, from Guiyu (e-waste-exposed group, n = 132) and Haojiang (reference group, n = 135) in November and December 2015. We measured child blood Pb, biomarkers including erythrocyte CD44 and CD58, erythrocyte count, leukocyte count and inflammatory cytokines (IL-1β, IL-12p70 and IFN-γ), and calculated erythrocyte Pb levels. Regression model demonstrated that higher erythrocyte Pb was associated with lower CD44 and CD58. Compared to low erythrocyte Pb levels (quartile 1), high erythrocyte Pb levels (quartile 4) were related to lower levels of erythrocyte CD44 and CD58. Elevated blood Pb correlated with higher IL-12p70 and IFN-γ, and lower IL-2. The mediation effect of erythrocyte CD44 on the relationship of erythrocyte Pb with IL-1β and IL-12p70 was significant, and the effect of erythrocyte Pb on IFN-γ was mediated by erythrocyte CD58. The data provides novel translational insight into the relationship between elevated Pb exposure and the change of erythrocyte immunity and downstream cytokine secretion in preschool children.
铅（Pb）毒性会损害血细胞并扰乱免疫微环境。当 Pb 进入循环系统时，超过 95%的 Pb 会积聚在红细胞中。因此，我们进行了这项研究，以探讨 Pb 暴露对红细胞黏附分子（CD44 和 CD58）表达及相关下游细胞因子浓度的长期影响。我们共纳入了 2015 年 11 月至 12 月来自贵屿（电子废物暴露组，n=132）和濠江（参照组，n=135）的 267 名 2-7 岁学龄前儿童。我们测量了儿童血 Pb、红细胞 CD44 和 CD58 等生物标志物、红细胞计数、白细胞计数和炎症细胞因子（IL-1β、IL-12p70 和 IFN-γ），并计算了红细胞 Pb 水平。回归模型表明，较高的红细胞 Pb 与较低的 CD44 和 CD58 相关。与低红细胞 Pb 水平（四分位 1）相比，高红细胞 Pb 水平（四分位 4）与红细胞 CD44 和 CD58 水平较低相关。血 Pb 升高与 IL-12p70 和 IFN-γ升高、IL-2 降低相关。红细胞 CD44 对红细胞 Pb 与 IL-1β 和 IL-12p70 关系的中介作用显著，红细胞 Pb 对 IFN-γ的作用则由红细胞 CD58 介导。这些数据为 Pb 暴露升高与学龄前儿童红细胞免疫改变和下游细胞因子分泌变化之间的关系提供了新的转化视角。
