碳硼（去硫）辅酶A（CH2CoA）衍生物在机理研究中的应用。

The use of carba(dethia)-coenzyme A (CH2CoA) derivatives for mechanistic investigations.

作者信息

Rétey J

机构信息

Institute of Organic Chemistry, University of Karlsruhe, Willstätter Allee, FRG.

出版信息

Biofactors. 1988 Dec;1(4):267-71.

PMID:3076442

Abstract

A novel class of acyl-coenzyme A analogues has been synthesized in which the sulphur atom is replaced by methylene. In contrast to their natural thiolester counterparts these acyl-CH2CoA analogues are stable to hydrolysis. They are good substrates for several enzymes that do not attack the thiolester group (carboxylases, mutases, dehydrogenases, epimerases, etc.) and potent inhibitors for most enzymes that do so. Some of the new insights gained by the use of acyl-CH2CoA are discussed in terms of enzymatic mechanisms.

摘要

一类新型的酰基辅酶A类似物已被合成出来，其中硫原子被亚甲基取代。与它们天然的硫酯对应物不同，这些酰基-CH₂CoA类似物对水解稳定。它们是几种不作用于硫酯基团的酶（羧化酶、变位酶、脱氢酶、表异构酶等）的良好底物，也是大多数作用于硫酯基团的酶的有效抑制剂。利用酰基-CH₂CoA所获得的一些新见解将根据酶促机制进行讨论。