Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, China.
Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, Gansu 730070, China; Tianjin Key Laboratory of Molecular Optoelectronics, Department of Chemistry, Tianjin University, Tianjin 300072, China.
J Chromatogr A. 2019 May 24;1593:1-8. doi: 10.1016/j.chroma.2019.02.005. Epub 2019 Feb 8.
Recently, water pollution caused by antibiotics is rapidly increasing. Thus, developing efficient, fast and sensitive detection methods for environmental antibiotics monitoring are still remaining elusive. Herein, a method for antibiotics analysis including lecofloxacin, pazcofloxacin and gatifloxacin in water by high performance liquid chromatography (HPLC) using molybdenum disulfide-graphene oxide-supported magnetic nanoparticles (FeO/GO/MoS) as the adsorbent of magnetic solid-phase extraction was developed. The as-prepared magnetic FeO/GO/MoS nanocomposite exhibited good enrichment capability toward fluoroquinolone antibiotics and the analytes were absorbed within a short time ca. 2 min. The main drive forces of FeO/GO/MoS nanocomposite and antibiotics were most likely attributed to hydrogen bonding and electrostatic attraction. A sensitive and effective MSPE-HPLC method was developed with low detection limits (LODs) ranging from 0.25 to 0.50 ng mL. The recoveries obtained from the analysis of water sample were between 85.6% and 106.1% with relative standard deviations (RSDs, n = 5) lower than 9.5%. The developed method has a good potential for the analysis of organic contaminants in water with low cost and high sensitivity. Therefore, this finding is a promising strategy for designing high efficiency and fast antibiotics detection system.
最近，抗生素引起的水污染迅速增加。因此，开发高效、快速和灵敏的环境抗生素监测检测方法仍然难以实现。在此，本文开发了一种通过高效液相色谱法（HPLC）分析水中包括左氧氟沙星、帕珠沙星和加替沙星在内的抗生素的方法，该方法使用二硫化钼-氧化石墨烯负载磁性纳米粒子（FeO/GO/MoS）作为磁性固相萃取的吸附剂。所制备的磁性 FeO/GO/MoS 纳米复合材料对氟喹诺酮类抗生素具有良好的富集能力，分析物在短时间内（约 2 分钟）被吸收。FeO/GO/MoS 纳米复合材料和抗生素的主要驱动力很可能归因于氢键和静电吸引。建立了一种灵敏有效的 MSPE-HPLC 方法，检测限（LOD）范围为 0.25-0.50ng/mL。水样分析的回收率在 85.6%到 106.1%之间，相对标准偏差（RSD，n=5）低于 9.5%。该方法具有低成本和高灵敏度分析水中有机污染物的良好潜力。因此，这一发现为设计高效快速抗生素检测系统提供了一种有前途的策略。
