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Tagging synthetic polymers with coumarin group for study nucleic acid interaction with gene delivery agents.

作者信息

Danilovtseva Elena N, Pal'shin Viktor A, Krishnan Uma M, Annenkov Vadim V, Zelinskiy Stanislav N

机构信息

Limnological Institute of the Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., P.O. Box 278, Irkutsk, 664033, Russia.

Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, Tamil Nadu, India.

出版信息

MethodsX. 2019 Jan 24;6:212-218. doi: 10.1016/j.mex.2019.01.008. eCollection 2019.

DOI:10.1016/j.mex.2019.01.008
PMID:30766801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6360603/
Abstract

Polymeric amines and complex amine containing system are actively studied and applied as gene delivery agents in gene therapy and genetic engineering. Optimizing polymer - nucleic acid ratio is the key stage in elaboration of procedures in this area. Application of fluorescent tagged oligonucleotides is widespread approach which allows to visualize nucleic acid in gel electrophoresis experiments and to find conditions of the full binding of the nucleic acid. We suggest to use succinimidyl ester of 7-(diethylamino)coumarin-3-carboxylic acid as an agent for fluorescent labeling of polymeric amines and to use the tagged polymers in optimizing polymer - nucleic acid ratio. This approach allows to see unbound polymer and to study various nucleic acids in interaction with the same polymer. •Labeling of gene delivery agents with fluorescence groups increases efficiency of optimization of gene delivery compositions.•Polymeric amines tagged with succinimidyl ester of 7-(diethylamino)coumarin-3-carboxylic acid are suitable for study polymer - nucleic acid interaction with gel electrophoresis.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/0aed64707b55/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/a8e20300b782/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/948f41734a28/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/5ed9c2eaf2a3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/42b94e76b56f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/6a36476b17d8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/0e45c8f6f8c3/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/0aed64707b55/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/a8e20300b782/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/948f41734a28/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/5ed9c2eaf2a3/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/42b94e76b56f/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/6a36476b17d8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/0e45c8f6f8c3/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/090c/6360603/0aed64707b55/gr6.jpg

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引用本文的文献

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本文引用的文献

1
Poly(vinyl amine)-silica composite nanoparticles: models of the silicic acid cytoplasmic pool and as a silica precursor for composite materials formation.聚（乙烯胺）-硅复合纳米粒子：硅质细胞液池模型和复合材料形成的硅前驱体。
Biomacromolecules. 2011 May 9;12(5):1772-80. doi: 10.1021/bm2001457. Epub 2011 Apr 21.
2
Synthesis of N epsilon-(7-diethylaminocoumarin-3-carboxyl)- and N epsilon-(7-methoxycoumarin-3-carboxyl)-L-Fmoc lysine as tools for protease cleavage detection by fluorescence.
J Pept Sci. 2005 Mar;11(3):153-60. doi: 10.1002/psc.608.
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A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.溴化乙锭与核酸之间的荧光复合物。物理化学特性
J Mol Biol. 1967 Jul 14;27(1):87-106. doi: 10.1016/0022-2836(67)90353-1.