Miami-Dade County Medical Examiner Department, Toxicology Laboratory, 1851 NW 10th Avenue, Miami FL, USA.
UF Health Pathology Laboratories-Forensic Toxicology Laboratory, Department of Pathology, Immunology, and Laboratory Medicine, University of Florida College of Medicine, 4800 SW 35th Drive, Gainesville FL, USA.
J Anal Toxicol. 2019 Jun 1;43(5):392-398. doi: 10.1093/jat/bky108.
In 2013, the Centers for Disease Control and Prevention released a warning regarding a new recreational drug, acetyl fentanyl. Acetyl fentanyl is a μ-opioid receptor agonist, and its pharmacological effects include euphoria, altered mood, miosis and central nervous system depression. The objective of this report was to develop a sensitive and specific method for the quantitation of acetyl fentanyl by gas chromatography-mass spectrometry in postmortem casework. Acetyl fentanyl was isolated from biological matrices using solid-phase extraction and acetyl fentanyl-13C6 was employed as an internal standard. The method was validated utilizing the Scientific Working Group for Forensic Toxicology's published method validation parameters, and the biological matrices used for analysis were postmortem blood and urine. In addition to the quantitation of acetyl fentanyl, a demographic study of cases obtained from the Rhode Island Office of State Medical Examiners and the University of Florida Health Pathology Laboratories-Forensic Toxicology Laboratory was performed to examine potential risk factors for acetyl fentanyl use. The results from this study found that the blood concentrations in these individuals ranged from 17 to 945 ng/mL. This suggests acetyl fentanyl is less potent than its prototype drug, fentanyl and requires an increased dose to achieve its desired effects. The demographic analysis indicated white males aged 21-40 years and individuals with a previous history of drug use have the highest risk for acetyl fentanyl abuse.
2013 年，疾病控制与预防中心发布了一份关于一种新型娱乐性药物乙酰芬太尼的警告。乙酰芬太尼是一种μ-阿片受体激动剂，其药理作用包括欣快、情绪改变、瞳孔缩小和中枢神经系统抑制。本报告的目的是开发一种灵敏、特异的气相色谱-质谱法检测死后检案中乙酰芬太尼的方法。乙酰芬太尼采用固相萃取法从生物基质中分离，并用乙酰芬太尼-13C6 作为内标。该方法利用科学工作组法医毒理学发布的方法验证参数进行了验证，用于分析的生物基质为死后血液和尿液。除了定量检测乙酰芬太尼外，还对从罗德岛州法医办公室和佛罗里达大学健康病理学实验室-法医毒理学实验室获得的案例进行了人口统计学研究，以检查使用乙酰芬太尼的潜在风险因素。这项研究的结果发现，这些个体的血液浓度范围为 17 至 945ng/mL。这表明乙酰芬太尼的效力不如其原型药物芬太尼，需要增加剂量才能达到预期的效果。人口统计学分析表明，21 至 40 岁的白人男性和有药物使用史的个体滥用乙酰芬太尼的风险最高。
