Mahmoudi Hassan, Pourhajibagher Maryam, Chiniforush Nasim, Soltanian Ali Reza, Alikhani Mohammad Yousef, Bahador Abbas
Department of Microbiology, Hamadan University of Medical Sciences, Hamadan, Iran.
Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran.
J Wound Care. 2019 Feb 2;28(2):66-73. doi: 10.12968/jowc.2019.28.2.66.
To investigate the relationship between biofilm formation and antibiotic resistance patterns in meticillin-resistant and meticillin-sensitive Staphylococcus aureus, isolated from burns.
In a cross-sectional study, pus/wound swab samples were obtained from burns. Presence of Staphylococcus aureus was confirmed, and biofilm formation-related icaABCDR and eta, etb genes were detected by polymerase chain reaction. Biofilm formation assay was assessed using the microtiter plate method. Antibiotic resistance was performed using the disk diffusion method and minimum inhibitory concentration.
A total of 95 patients with burns were recruited. Of the 95 wounds swabbed, Staphylococcus aureus was identified in 50 (62.5%), and 47 (94%) isolates capable of producing biofilm. Biofilm production levels were classed as 'strong' (n=29; 58%), 'moderate' (n=11; 22%), 'weak' (n=7; 14%) and 'non-biofilm forming' (n=3; 6%). There was an almost even split between isolates identified as meticillin-resistant Staphylococcus aureus (MRSA), n=24 (48%), and meticillin-sensitive Staphylococcus aureus (MSSA) n=26 (52%). The prevalence of the icaA, icaB, icaC, icaD and icaR genes among the studied isolates were 96%, 80%, 80%, 96% and 84%, respectively. The prevalence of eta and etb genes in isolates were 84% and 92%, respectively.
Biofilm producing isolates of Staphylococcus aureus showed greater multidrug resistance than non-biofilm producers. In our study, a high rate of biofilm formation and antimicrobial drug resistance was seen. Our results highlight the alarming levels of antimicrobial resistance among MRSA strains and important data about the prevalence of eta and etb genes in Staphylococcus aureus strains isolated from burn patients in this study.
研究从烧伤患者中分离出的耐甲氧西林金黄色葡萄球菌和甲氧西林敏感金黄色葡萄球菌的生物膜形成与抗生素耐药模式之间的关系。
在一项横断面研究中，从烧伤患者处获取脓液/伤口拭子样本。确认金黄色葡萄球菌的存在，并通过聚合酶链反应检测与生物膜形成相关的icaABCDR以及eta、etb基因。使用微量滴定板法评估生物膜形成试验。采用纸片扩散法和最低抑菌浓度法检测抗生素耐药性。
共招募了95名烧伤患者。在擦拭的95个伤口中，50个（62.5%）鉴定出金黄色葡萄球菌，其中47个（94%）分离株能够产生生物膜。生物膜产生水平分为“强”（n = 29；58%）、“中度”（n = 11；22%）、“弱”（n = 7；14%）和“非生物膜形成”（n = 3；6%）。鉴定为耐甲氧西林金黄色葡萄球菌（MRSA）的分离株有24个（48%），甲氧西林敏感金黄色葡萄球菌（MSSA）的分离株有26个（52%），两者几乎平分。在所研究的分离株中，icaA、icaB、icaC、icaD和icaR基因的流行率分别为96%、80%、80%、96%和84%。分离株中eta和etb基因的流行率分别为84%和92%。
产生生物膜的金黄色葡萄球菌分离株比不产生生物膜的分离株表现出更强的多重耐药性。在我们的研究中，观察到生物膜形成率和抗菌药物耐药率都很高。我们的结果凸显了MRSA菌株中令人担忧的抗菌药物耐药水平，以及本研究中从烧伤患者分离出的金黄色葡萄球菌菌株中eta和etb基因流行率的重要数据。
