Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07 Nitra, Slovakia; Mykola Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska 2, 01004 Kyiv, Ukraine.
Institute of Plant Genetics and Biotechnology, Plant Science and Biodiversity Center, Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07 Nitra, Slovakia.
Ecotoxicol Environ Saf. 2019 May 30;173:86-95. doi: 10.1016/j.ecoenv.2019.02.012. Epub 2019 Feb 12.
Large areas polluted with toxic heavy metals or radionuclides were formed as a side product of rapid industrial development of human society. Plants, due to their sessile nature, should adapt to these challenging genotoxic environmental conditions and develop resistance. Herein, we evaluated the response of three natural ecotypes of Arabidopsis thaliana (L.) Heynh (Oasis, Columbia-0, and Chernobyl-07) to cadmium, using discovery gel-based proteomics. These accessions are differing by level of tolerance to heavy metal probably achieved by various exposure to chronic ionizing radiation. Based on the pairwise comparison (control versus cadmium-treated) we recognized 5.8-13.4% of identified proteins as significantly altered at the presence of cadmium. Although the majority of photosynthesis-related proteins were found to be less abundant in all ecotypes it was noted that in contrast to the sensitive variants (Col and Oas), the tolerant Che accession may activate the mechanism preserving photosynthesis and energy production. Also, proteins modulating energy budget through alternative route and mediating higher resistance to heavy metals were upregulated in this ecotype. Although we suggest that regulation of enzymes acting in peptide and protein synthesis, protection of the plants against various abiotic stresses, or those neutralizing the effects of reactive oxygen species are rather associated with general response to cadmium, they were found to be altered more intensively in the Che accession. Thus, the identified affected proteins may represent good candidate molecules for molecular breeding to improve tolerance of crops to heavy metal stress.
大面积被有毒重金属或放射性核素污染的地区是人类社会快速工业化发展的副产品。由于植物的固着特性，它们应该适应这些具有遗传毒性的挑战性环境条件并发展抗性。在这里，我们使用基于发现凝胶的蛋白质组学评估了三种拟南芥（Arabidopsis thaliana）天然生态型（绿洲、哥伦比亚-0 和切尔诺贝利-07）对镉的反应。这些品系通过不同程度的耐受重金属，可能是通过各种慢性电离辐射暴露而实现的。基于两两比较（对照与镉处理），我们发现有 5.8-13.4%的鉴定蛋白在镉存在时明显改变。尽管大多数与光合作用相关的蛋白质在所有生态型中都较少，但值得注意的是，与敏感变体（Col 和 Oas）相比，耐受的 Che 品系可能会激活保护光合作用和能量产生的机制。此外，通过替代途径调节能量预算和介导对重金属更高抗性的蛋白质在这个生态型中上调。虽然我们认为调节参与肽和蛋白质合成的酶、保护植物免受各种非生物胁迫、或中和活性氧的影响可能与对镉的一般反应有关，但它们在 Che 品系中被发现改变得更为剧烈。因此，鉴定出的受影响蛋白质可能是用于分子育种的良好候选分子，以提高作物对重金属胁迫的耐受性。
