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泡菜和 泡菜中生物胺的产生及产生物胺乳酸菌的测定 。 注:原文中“and”前后似乎有缺失内容,不太完整。

The Occurrence of Biogenic Amines and Determination of Biogenic Amine-Producing Lactic Acid Bacteria in and Kimchi.

作者信息

Jin Young Hun, Lee Jae Hoan, Park Young Kyung, Lee Jun-Hee, Mah Jae-Hyung

机构信息

Department of Food and Biotechnology, Korea University, 2511 Sejong-ro, Sejong 30019, Korea.

出版信息

Foods. 2019 Feb 14;8(2):73. doi: 10.3390/foods8020073.

Abstract

In this study, biogenic amine content in two types of fermented radish kimchi ( and kimchi) was determined by high performance liquid chromatography (HPLC). While most samples had low levels of biogenic amines, some samples contained histamine content over the toxicity limit. Additionally, significant amounts of total biogenic amines were detected in certain samples due to high levels of putrefactive amines. As one of the significant factors influencing biogenic amine content in both radish kimchi, appeared to be important source of histamine. Besides, tyramine-producing strains of lactic acid bacteria existed in both radish kimchi. Through 16s rRNA sequencing analysis, the dominant species of tyramine-producing strains was identified as , which suggests that the species is responsible for tyramine formation in both radish kimchi. During fermentation, a higher tyramine accumulation was observed in both radish kimchi when strains were used as inocula. The addition of affected the initial concentrations of histamine and cadaverine in both radish kimchi. Therefore, this study suggests that reducing the ratio of to other ingredients (and/or using with low biogenic amine content) and using starter cultures with ability to degrade and/or inability to produce biogenic amines would be effective in reducing biogenic amine content in and kimchi.

摘要

在本研究中,通过高效液相色谱法(HPLC)测定了两种发酵萝卜泡菜([具体种类1]泡菜和[具体种类2]泡菜)中的生物胺含量。虽然大多数样品的生物胺含量较低,但一些样品中的组胺含量超过了毒性限值。此外,由于腐胺含量较高,在某些样品中检测到了大量的总生物胺。作为影响两种萝卜泡菜生物胺含量的重要因素之一,[具体因素]似乎是组胺的重要来源。此外,两种萝卜泡菜中都存在产酪胺的乳酸菌菌株。通过16s rRNA测序分析,产酪胺菌株的优势种被鉴定为[具体菌种],这表明该菌种是两种萝卜泡菜中酪胺形成的原因。在发酵过程中,当使用[具体菌株]作为接种物时,两种萝卜泡菜中均观察到较高的酪胺积累。[具体物质]的添加影响了两种萝卜泡菜中组胺和尸胺的初始浓度。因此,本研究表明,降低[具体物质]与其他成分的比例(和/或使用生物胺含量低的[具体物质])以及使用具有降解能力和/或不产生生物胺能力的发酵剂将有效地降低[具体种类1]泡菜和[具体种类2]泡菜中的生物胺含量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b4/6406508/403db57aa3b0/foods-08-00073-g001.jpg

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