Department of Food Science and Nutrition, Pusan National University, Busan 46241, Korea.
Division of Marine Bioscience, Korea Maritime and Ocean University, Busan 49112, Korea.
Nutrients. 2019 Feb 14;11(2):404. doi: 10.3390/nu11020404.
In this study, we investigated whether ()-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone, a homoisoflavonoid compound isolated from L., protects INS-1 pancreatic β cells against glucotoxicity-induced apoptosis. Treatment with high glucose (30 mM) induced apoptosis in INS-1 pancreatic β cells; however, the level of cell viability was significantly increased by treatment with ()-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone. Treatment with 10⁻20 µM of ()-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone dose-dependently increased cell viability and significantly decreased the intracellular level of reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), and nitric oxide levels in INS-1 pancreatic β cells pretreated with high glucose. These effects were associated with increased anti-apoptotic Bcl-2 protein expression, while reducing pro-apoptotic Bax, cytochrome C, and caspase 9 protein expression. Treatment with ()-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone reduced the apoptosis previously induced by high-level glucose-treatment, according to annexin V/propidium iodide staining. These results demonstrate that ()-5-hydroxy-7-methoxy-3-(2'-hydroxybenzyl)-4-chromanone may be useful as a potential therapeutic agent to protect INS-1 pancreatic β cells against high glucose-induced apoptosis.
在这项研究中，我们研究了从 中分离得到的同型异黄酮化合物()-5-羟基-7-甲氧基-3-(2'-羟基苄基)-4-色满酮是否可以保护 INS-1 胰腺β细胞免受糖毒性诱导的细胞凋亡。高浓度葡萄糖（30mM）处理可诱导 INS-1 胰腺β细胞凋亡；然而，用()-5-羟基-7-甲氧基-3-(2'-羟基苄基)-4-色满酮处理可显著提高细胞活力。用 10-20μM 的()-5-羟基-7-甲氧基-3-(2'-羟基苄基)-4-色满酮进行剂量依赖性处理，可增加细胞活力，并显著降低高糖预处理的 INS-1 胰腺β细胞内活性氧（ROS）、硫代巴比妥酸反应物质（TBARS）和一氧化氮水平。这些作用与抗凋亡 Bcl-2 蛋白表达增加有关，同时降低促凋亡 Bax、细胞色素 C 和 caspase 9 蛋白表达。用()-5-羟基-7-甲氧基-3-(2'-羟基苄基)-4-色满酮处理可减少先前由高浓度葡萄糖处理诱导的细胞凋亡，根据 Annexin V/碘化丙啶染色。这些结果表明，()-5-羟基-7-甲氧基-3-(2'-羟基苄基)-4-色满酮可用作一种潜在的治疗剂，以保护 INS-1 胰腺β细胞免受高葡萄糖诱导的细胞凋亡。
