Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung 201-340; Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung 210-702, Korea.
College of Pharmacy, Hanyang University, Ansan 426-791, Korea.
BMB Rep. 2015 May;48(5):289-94. doi: 10.5483/bmbrep.2015.48.5.189.
Caesalpinia sappan is a well-distributed plant that is cultivated in Southeast Asia, Africa, and the Americas. C. sappan has been used in Asian folk medicine and its extract has been shown to have pharmacological effects. Two homoisoflavonoids, sappanol and brazilin, were isolated from C. sappan by using centrifugal partition chromatography (CPC), and tested for protective effects against retinal cell death. The isolated homoisoflavonoids produced approximately 20-fold inhibition of N-retinylidene-N-retinyl-ethanolamine (A2E) photooxidation in a dose-dependent manner. Of the 2 compounds, brazilin showed better inhibition (197.93 ± 1.59 μM of IC50). Cell viability tests and PI/Hoechst 33342 double staining method indicated that compared to the negative control, sappanol significantly attenuated H2O2-induced retinal death. The compounds significantly blunted the up-regulation of intracellular reactive oxygen species (ROS), and sappanol inhibited lipid peroxidation in a concentration-dependent manner. Thus, both compounds represent potential antioxidant treatments for retinal diseases.
苏木是一种分布广泛的植物，分布于东南亚、非洲和美洲。苏木在亚洲民间医学中被使用，其提取物具有药理学作用。使用离心分配色谱（CPC）从苏木中分离出两种异黄酮，即苏木醇和巴西红，并用其来测试对视网膜细胞死亡的保护作用。分离出的异黄酮对 N-视黄醛基-N-视黄醇基乙胺（A2E）的光氧化具有约 20 倍的抑制作用，呈剂量依赖性。在这两种化合物中，巴西红显示出更好的抑制作用（IC50 为 197.93±1.59μM）。细胞活力测试和 PI/Hoechst 33342 双重染色法表明，与阴性对照相比，苏木醇显著减轻了 H2O2 诱导的视网膜死亡。这些化合物显著减轻了细胞内活性氧（ROS）的上调，并且苏木醇以浓度依赖性方式抑制脂质过氧化。因此，这两种化合物均代表用于治疗视网膜疾病的潜在抗氧化治疗药物。
