Ryerson University, Department of Physics, Faculty of Science, Toronto, Canada.
Institute for Biomedical Engineering, Science and Technology, Li Ka Shing Knowledge Institute, St. M, Canada.
J Biomed Opt. 2019 Feb;24(2):1-7. doi: 10.1117/1.JBO.24.2.025002.
Optical coherence tomography (OCT) is used to provide anatomical information of biological systems but can also provide functional information by characterizing the motion of intracellular structures. Dynamic light scattering OCT was performed on intact, control MCF-7 breast cancer cells and cells either treated with paclitaxel to induce apoptosis or deprived of nutrients to induce oncosis. Autocorrelations (ACs) of the temporal fluctuations of OCT intensity signals demonstrate a significant decrease in decorrelation time after 24 h in both the paclitaxel-treated and nutrient-deprived cell groups but no significant differences between the two groups. The acquired ACs were then used as input for the CONTIN deconvolution algorithm, and all produced CONTIN outputs with three distinct peaks for all experimental conditions. After 24 h of either paclitaxel treatment or nutrient deprivation, the area-under-the-curve (AUC) of the first peak increased significantly while the AUC of the third peak decreased significantly. These results lend strong support to the hypothesis that ACs acquired from cells are composed of multiple components that correspond to light scattered by different subcellular structures and organelles.
光学相干断层扫描（OCT）用于提供生物系统的解剖学信息，但也可以通过描述细胞内结构的运动来提供功能信息。对完整的、对照的 MCF-7 乳腺癌细胞以及用紫杉醇处理以诱导细胞凋亡或剥夺营养物质以诱导细胞胀亡的细胞进行动态光散射 OCT 分析。OCT 强度信号的时间波动的自相关（AC）表明，紫杉醇处理组和营养剥夺组的去相关时间在 24 小时后均显著降低，但两组之间无显著差异。然后将获得的 AC 用作 CONTIN 反卷积算法的输入，并且对于所有实验条件，所有产生的 CONTIN 输出都具有三个明显的峰。在紫杉醇处理或营养剥夺 24 小时后，第一峰的曲线下面积（AUC）显著增加，而第三峰的 AUC 显著降低。这些结果有力地支持了这样的假设，即从细胞中获得的 AC 由多个对应于不同亚细胞结构和细胞器散射光的分量组成。
