Lu Mengli, Lv Liying
Clinical Laboratory, First Affiliated Hospital, Anhui Medical University, Hefei, People's Republic of China.
Lab Med. 2019 Jul 16;50(3):273-278. doi: 10.1093/labmed/lmy086.
To analyze the performance characteristics, stability, and clinical value of lipoprotein-associated phospholipase A2 (Lp-PLA2) using an enzymatic kinetic method.
The performance characteristics included reference intervals, precision, and accuracy. We assessed Lp-PLA2 stability by comparing Lp-PLA2 changes under different conditions. Lp-PLA2 was determined in the following groups: control individuals, patients with coronary heart disease (CHD), patients of different lipid subgroups within CHD, and patients with high total cholesterol (TC). Also, correlations between Lp-PLA2 and traditional cardiovascular risk factors were assessed.
The mean (SD) reference interval of serum Lp-PLA2 activity was 451 (113) U per L with sex differences. Inter- and intra-assay precision revealed coefficients of variance (CVs) of 1.81% to 2.63% and 1.43% to 1.77%. The average bias was 0.33%. Lp-PLA2 activity was stable. In the CHD group, high-lipid subgroups, and high-TC group, Lp-PLA2 was elevated, and correlation was observed between Lp-PLA2 and traditional risk factors.
Lp-PLA2 activity has important clinical value in CHD.
采用酶动力学方法分析脂蛋白相关磷脂酶A2（Lp-PLA2）的性能特征、稳定性及临床价值。
性能特征包括参考区间、精密度和准确性。通过比较不同条件下Lp-PLA2的变化来评估其稳定性。在以下几组中测定Lp-PLA2：对照组个体、冠心病（CHD）患者、CHD内不同血脂亚组的患者以及高总胆固醇（TC）患者。此外，还评估了Lp-PLA2与传统心血管危险因素之间的相关性。
血清Lp-PLA2活性的平均（标准差）参考区间为每升451（113）U，存在性别差异。批间和批内精密度显示变异系数（CV）为1.81%至2.63%和1.43%至1.77%。平均偏差为0.33%。Lp-PLA2活性稳定。在CHD组、高脂亚组和高TC组中，Lp-PLA2升高，且Lp-PLA2与传统危险因素之间存在相关性。
Lp-PLA2活性在CHD中具有重要的临床价值。
