酶动力学法检测脂蛋白相关磷脂酶A2的性能特征及临床价值
Performance Characteristics and Clinical Value of the Lipoprotein-Associated Phospholipase A2 by an Enzymatic Kinetic Method.
作者信息
Lu Mengli, Lv Liying
机构信息
Clinical Laboratory, First Affiliated Hospital, Anhui Medical University, Hefei, People's Republic of China.
出版信息
Lab Med. 2019 Jul 16;50(3):273-278. doi: 10.1093/labmed/lmy086.
OBJECTIVE
To analyze the performance characteristics, stability, and clinical value of lipoprotein-associated phospholipase A2 (Lp-PLA2) using an enzymatic kinetic method.
METHODS
The performance characteristics included reference intervals, precision, and accuracy. We assessed Lp-PLA2 stability by comparing Lp-PLA2 changes under different conditions. Lp-PLA2 was determined in the following groups: control individuals, patients with coronary heart disease (CHD), patients of different lipid subgroups within CHD, and patients with high total cholesterol (TC). Also, correlations between Lp-PLA2 and traditional cardiovascular risk factors were assessed.
RESULTS
The mean (SD) reference interval of serum Lp-PLA2 activity was 451 (113) U per L with sex differences. Inter- and intra-assay precision revealed coefficients of variance (CVs) of 1.81% to 2.63% and 1.43% to 1.77%. The average bias was 0.33%. Lp-PLA2 activity was stable. In the CHD group, high-lipid subgroups, and high-TC group, Lp-PLA2 was elevated, and correlation was observed between Lp-PLA2 and traditional risk factors.
CONCLUSION
Lp-PLA2 activity has important clinical value in CHD.
目的
采用酶动力学方法分析脂蛋白相关磷脂酶A2（Lp-PLA2）的性能特征、稳定性及临床价值。
方法
性能特征包括参考区间、精密度和准确性。通过比较不同条件下Lp-PLA2的变化来评估其稳定性。在以下几组中测定Lp-PLA2：对照组个体、冠心病（CHD）患者、CHD内不同血脂亚组的患者以及高总胆固醇（TC）患者。此外，还评估了Lp-PLA2与传统心血管危险因素之间的相关性。
结果
血清Lp-PLA2活性的平均（标准差）参考区间为每升451（113）U，存在性别差异。批间和批内精密度显示变异系数（CV）为1.81%至2.63%和1.43%至1.77%。平均偏差为0.33%。Lp-PLA2活性稳定。在CHD组、高脂亚组和高TC组中，Lp-PLA2升高，且Lp-PLA2与传统危险因素之间存在相关性。
结论
Lp-PLA2活性在CHD中具有重要的临床价值。
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