Trace Analysis and Biosensor Research Center, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Center of Excellence for Innovation in Chemistry, Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Singapore; NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, University Hall, Tan Chin Tuan Wing #04-02 21 Lower Kent Ridge Road 119077, Singapore.
Talanta. 2019 May 15;197:465-471. doi: 10.1016/j.talanta.2019.01.046. Epub 2019 Jan 12.
An environmentally friendly method of etching a stainless steel plunger wire, to replace the conventional hydrofluoric acid approach, was developed for plunger-in-needle liquid phase-microextraction (PIN-LPME). The one-step etching procedure was performed by immersing the plunger wire in a ferric chloride-hydrochloric acid (FeCl-HCl) solution. The etched wire was then used for LPME as the organic solvent holder. After solvent coating, the wire was directly exposed to a water sample for extraction of polycyclic aromatic hydrocarbons (PAHs), which were then subjected to thermal desorption in the injector of a gas chromatograph for gas chromatography-mass spectrometric (GC-MS) analysis. The parameters affecting PIN-LPME efficiencies (i.e., extraction solvent, solvent coating mode and time, stirring rate, extraction time and salting effect) were also investigated. Under the most favourable conditions, PIN-LPME-GC-MS exhibited high enrichment factors of between 70 and 349 for the 9 PAHs, low detection limits (between 0.006 and 0.058 ng mL), and good precision (with relative standard deviations ranging from 4.4% to 9.7%). The developed method was successfully applied for the extraction and determination of PAHs in tap, river and drain water samples. Good relative recoveries of the PAHs over the range of 84.3-101.9% were obtained with spiked genuine water samples.
开发了一种环保的不锈钢柱塞线蚀刻方法，以替代传统的氢氟酸方法，用于柱塞进针液相微萃取（PIN-LPME）。一步蚀刻过程是将柱塞线浸入氯化铁-盐酸（FeCl-HCl）溶液中完成的。然后，将蚀刻后的线用作 LPME 的有机溶剂固定器。在溶剂涂层后，将线直接暴露于水样中，以萃取多环芳烃（PAHs），然后在气相色谱仪的进样器中进行热解吸，用于气相色谱-质谱联用（GC-MS）分析。还研究了影响 PIN-LPME 效率的参数（即萃取溶剂、溶剂涂层模式和时间、搅拌速度、萃取时间和盐效应）。在最有利的条件下，PIN-LPME-GC-MS 对 9 种 PAHs 的富集因子在 70 至 349 之间，检测限低（在 0.006 至 0.058 ng/mL 之间），精密度良好（相对标准偏差在 4.4%至 9.7%之间）。该方法成功地应用于自来水中、河水和污水中 PAHs 的提取和测定。用真实水样加标，可获得 PAHs 的相对回收率在 84.3%至 101.9%范围内。
