Nishimura Kanae, Haginaka Jun
Department of Pharmacy, School of Pharmacy and Pharmaceutical Sciences, Mukogawa Women's University.
Institute for Biosciences, Mukogawa Women's University.
Anal Sci. 2019 Jun 10;35(6):659-664. doi: 10.2116/analsci.19P011. Epub 2019 Feb 15.
Molecularly imprinted polymers (MIPs) for promazine (PZ) and chlorpromazine (CPZ), MIP and MIP, were prepared by multi-step swelling and polymerization using methacrylic acid as a functional monomer and ethylene glycol dimethacrylate as a crosslinker. The retention and molecular-recognition properties of MIP and MIP were evaluated using a mixture of potassium phosphate buffer and acetonitrile, or a mixture of ammonium formate and acetonitrile as the mobile phase in LC. PZ and CPZ gave the maximal retentions on MIP and MIP at an apparent pH 8.2 using a mixture of potassium phosphate buffer and acetonitrile as the mobile phase. The retentions of PZ and CPZ decreased with an increase of acetonitrile contents from 70 to 90 vol% using a mixture of ammonium formate and acetonitrile as the mobile phase. The template molecules (PZ and CPZ, respectively) were recognized the most on the respective MIPs, and the imprinting factor of PZ was higher on MIP than on MIP. These results indicate that in addition to shape recognition, ionic and hydrophobic interactions seem to work for the retention and molecular-recognition of PZ and CPZ on the MIPs. MIP was successfully utilized for the selective extraction of PZ in rat-serum samples in column-switching LC with fluorescence detection.
以甲基丙烯酸为功能单体、乙二醇二甲基丙烯酸酯为交联剂，通过多步溶胀聚合制备了用于丙嗪（PZ）和氯丙嗪（CPZ）的分子印迹聚合物（MIP），即MIP和MIP。在液相色谱中，以磷酸钾缓冲液和乙腈的混合物或甲酸铵和乙腈的混合物为流动相，评估了MIP和MIP的保留和分子识别特性。以磷酸钾缓冲液和乙腈的混合物为流动相时，PZ和CPZ在表观pH 8.2时在MIP和MIP上的保留时间最长。以甲酸铵和乙腈的混合物为流动相时，随着乙腈含量从70%增加到90%（体积分数），PZ和CPZ的保留时间缩短。模板分子（分别为PZ和CPZ）在各自的MIP上识别能力最强，PZ在MIP上的印迹因子高于在MIP上的印迹因子。这些结果表明，除了形状识别外，离子和疏水相互作用似乎也对PZ和CPZ在MIP上的保留和分子识别起作用。在柱切换液相色谱-荧光检测中，MIP成功用于大鼠血清样品中PZ的选择性萃取。
