通过可逆加成-断裂链转移聚合（RAFT）合成的支链聚（丙烯酸乙酯三甲基鏻——PEGA）：用于核酸络合的阳离子聚铵替代品。

Cook Alexander B, Peltier Raoul, Barlow Tammie R, Tanaka Joji, Burns James A, Perrier Sébastien

Department of Chemistry University of Warwick Coventry CV4 7AL UK.

Syngenta, Jealott's Hill International Research Centre, Bracknell Berkshire RG42 6EY UK.

J Interdiscip Nanomed. 2018 Dec 21;3(4):164-174. doi: 10.1002/jin2.50. eCollection 2018 Dec.

Cationic and highly branched poly (trimethylphosphonium ethylacrylate--poly (ethylene glycol) acrylate) (p (TMPEA--PEGA)), and its ammonium equivalent, have been synthesised from post-polymerisation modification of a poly (bromo ethylacrylate--poly (ethylene glycol) acrylate) (p (BEA--PEGA)) precursor polymer produced using reversible addition fragmentation chain transfer (RAFT) polymerisation. The cationic polymers were evaluated for their ability to complex nucleic acids, their i cytotoxicity and their GFP pDNA transfection efficiency. The results show RAFT copolymerisation of BEA and PEGA is a simple route to polyphosphoniums showing reduced cytotoxicities and higher transfection efficiencies than their polyammonium alternatives.

阳离子型且高度支化的聚（丙烯酸乙酯三甲基鏻-聚（乙二醇）丙烯酸酯）（p（TMPEA-PEGA））及其铵类等效物，是通过对采用可逆加成断裂链转移（RAFT）聚合制备的聚（溴代丙烯酸乙酯-聚（乙二醇）丙烯酸酯）（p（BEA-PEGA））前体聚合物进行后聚合改性而合成的。对这些阳离子聚合物的核酸络合能力、细胞毒性及绿色荧光蛋白质粒DNA转染效率进行了评估。结果表明，BEA和PEGA的RAFT共聚是制备聚鏻盐的一种简单方法，与它们的聚铵类替代物相比，其细胞毒性降低，转染效率更高。