从出生后的斯普拉格-道利大鼠中分离外毛细胞。

Outer hair cells isolation from postnatal Sprague-Dawley rats.

作者信息

Qi Mei-Hao, Qiu Yang, Tian Ke-Yong, Liang Kun, Chang Hui-Min, Wang Ren-Feng, Chen Er-Fang, Wang Wei-Long, Zha Ding-Jun, Qiu Jian-Hua

机构信息

Department of Otorhinolaryngology Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, PR China.

Department of Otorhinolaryngology Head and Neck Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, PR China.

出版信息

World J Otorhinolaryngol Head Neck Surg. 2019 Jan 11;5(1):14-18. doi: 10.1016/j.wjorl.2018.01.001. eCollection 2019 Mar.

DOI:10.1016/j.wjorl.2018.01.001

PMID:30775696

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6364511/

Abstract

Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing loss. In order to elucidate the mechanism underlying these disorders, OHCs - its diseased region needs to be deeply investigated. However, OHCs array on the basilar membrane which contains massive cells with different types. Therefore, to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease processing. In the present study, we tried to isolate OHCs from the commonly used animal model -Sprague-Dawley (SD) rats. By separating outer hair cells from SD rats with different day ages, we found that 9 days after birth was a suitable time for the separation of the OHCs. At this time, the number of OHCs isolated from rats was large, and the cell morphology was typical of cylindrical shape. OHCs isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses.

摘要

外毛细胞（OHCs）损伤是噪声性听力损失和药物性听力损失等临床疾病中的常见现象。为了阐明这些疾病的潜在机制，需要深入研究OHCs及其病变区域。然而，OHCs排列在基底膜上，基底膜包含大量不同类型的细胞。因此，从这一庞大细胞群体中分离出OHCs对于揭示其在疾病过程中的病理和分子变化具有重要意义。在本研究中，我们试图从常用的动物模型——Sprague-Dawley（SD）大鼠中分离出OHCs。通过分离不同日龄SD大鼠的外毛细胞，我们发现出生后9天是分离OHCs的合适时间。此时，从大鼠中分离出的OHCs数量较多，细胞形态呈典型的圆柱形。用这种方法分离出的OHCs在组织学上适合且在数量上足以进行分子生物学和电生理分析。