Hegde Shruthi, Ajila Vidya, Babu Subhas, Kumari Suchetha, Ullal Harshini, Madiyal Ananya
Department of Oral Medicine and Radiology, A B Shetty Memorial Institute of Dental Sciences, Nitte University, Mangalore, India.
Department of Biochemistry, K.S. Hegde Medical Academy, Nitte University, Mangalore, India.
Eur Oral Res. 2018 Sep;52(3):157-161. doi: 10.26650/eor.2018.543. Epub 2018 Sep 1.
Present study was undertaken to evaluate and compare the salivary levels of tumour necrosis factor alpha (TNF-α) in subjects with RAS, traumatic ulcers (TUs) in the oral mucosa and in healthy controls.
Present study involved 90 participants of which 30 subjects were diagnosed with RAS, 30 subjects with TUs and 30 healthy controls grouped as group 1, group 2 and group 3 respectively. Unstimulated saliva was collected from the subjects through 'Spit Technique' and the estimation of TNF-α was done by enzyme linked immunosorbent assay. The data collected was statistically analysed.
Salivary level of TNF-α was significantly higher in RAS patients than in patients with TUs and healthy controls. Difference between the Salivary TNF-α level in our study groups were statistically significant (p<0.001).
Present study suggests that saliva is a convenient and ideal medium for the detection of TNF-α. Statistically significant difference in the level of salivary TNF-α between the RAS and TUs subjects as well as controls suggests the significant contribution of TNF-α in pathogenesis of RAS.
本研究旨在评估和比较复发性口疮性口炎（RAS）患者、口腔黏膜创伤性溃疡（TUs）患者及健康对照者唾液中肿瘤坏死因子α（TNF-α）的水平。
本研究纳入90名参与者，其中30名被诊断为RAS，30名患有TUs，30名健康对照者分别作为第1组、第2组和第3组。通过“吐唾技术”从受试者中收集非刺激性唾液，并采用酶联免疫吸附测定法对TNF-α进行检测。对收集到的数据进行统计学分析。
RAS患者唾液中TNF-α水平显著高于TUs患者和健康对照者。我们研究组之间唾液TNF-α水平的差异具有统计学意义（p<0.001）。
本研究表明，唾液是检测TNF-α的便捷且理想的介质。RAS患者与TUs患者及对照者之间唾液TNF-α水平存在统计学显著差异，提示TNF-α在RAS发病机制中具有重要作用。
