Novák Ondřej, Pěnčík Aleš, Blahoušek Ota, Ljung Karin
Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany CAS, Faculty of Science, Palacký University, Olomouc, Czech Republic.
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.
Curr Protoc Plant Biol. 2016 Mar;1(2):419-430. doi: 10.1002/cppb.20028.
Auxin, a plant hormone, is able to stimulate growth and to control developmental processes. To get a deeper level of understanding of how auxin activity is regulated in planta, we have developed a sensitive and selective mass spectrometry-based protocol for multiplex quantification of indole 3-acetic acid (IAA) as well as its precursors and degradation products. As part of the protocol development, we have also established a derivatization protocol for the IAA precursors IPyA and IAAld, compounds that are highly labile during sample extraction and purification. This article describes a standard isotope dilution method for quantitative profiling of the IAA metabolome in small amounts of plant tissues. © 2016 by John Wiley & Sons, Inc.
生长素作为一种植物激素，能够刺激生长并控制发育过程。为了更深入地了解植物体内生长素活性是如何被调控的，我们开发了一种基于质谱的灵敏且具选择性的方法，用于对吲哚 - 3 - 乙酸（IAA）及其前体和降解产物进行多重定量分析。作为方法开发的一部分，我们还建立了针对IAA前体IPyA和IAAld的衍生化方法，这两种化合物在样品提取和纯化过程中极不稳定。本文描述了一种用于对少量植物组织中IAA代谢组进行定量分析的标准同位素稀释法。© 2016约翰威立父子出版公司
