Crocoll Christoph, Halkier Barbara Ann, Burow Meike
DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Copenhagen, Denmark.
Curr Protoc Plant Biol. 2016 Mar;1(2):385-409. doi: 10.1002/cppb.20027.
Recent advances in liquid chromatography and mass spectrometry have made it possible to increase the throughput of phytochemical analyses at high sensitivity. These improvements have made it more feasible to monitor metabolic processes at the metabolite level. Glucosinolates, the primary defense compounds in the model plant Arabidopsis thaliana, in particular, have gained increasing attention as model compounds for quantitative genetics and metabolic regulation. Depending on the plant species, tissue, glucosinolate content, complexity of the glucosinolate profile, and, most importantly, the overall purpose of the experiment, different choices need to be made regarding the methods of extraction and analysis. In this chapter, we describe different approaches for the analysis of glucosinolates and highlight advantages, disadvantages, and technical pitfalls. © 2016 by John Wiley & Sons, Inc.
液相色谱和质谱技术的最新进展使得高灵敏度地提高植物化学分析的通量成为可能。这些改进使得在代谢物水平监测代谢过程变得更加可行。特别是，芥子油苷作为模式植物拟南芥中的主要防御化合物，作为定量遗传学和代谢调控的模式化合物越来越受到关注。根据植物种类、组织、芥子油苷含量、芥子油苷谱的复杂性，以及最重要的是实验的总体目的，需要对提取和分析方法做出不同的选择。在本章中，我们描述了分析芥子油苷的不同方法，并突出了其优点、缺点和技术陷阱。© 2016约翰威立国际出版公司
