高剂量生物素补充剂对利用链霉亲和素与生物素相互作用的免疫测定中甲状腺参数的干扰：一例报告及当前文献综述
Interference of High Dose Biotin Supplementation with Thyroid Parameters in Immunoassays Utilizing the Interaction between Streptavidin and Biotin: a Case Report and Review of Current Literature.
作者信息
Mrosewski Ingo, Neumann Ilka, Switkowski Rafael
出版信息
Clin Lab. 2019 Jan 1;65(1). doi: 10.7754/Clin.Lab.2018.180637.
BACKGROUND
Automated immunoassays utilizing the interaction between streptavidin and biotin are widely used. Nonetheless, biotin remains an often overlooked confounder.
METHODS
We report the case of a 54-year-old female patient with progressive multiple sclerosis and Hashimoto's thyroiditis who presented herself for a follow-up. Measurements on Roche's cobas® 8000 modular analyzer series suggested severe hyperthyroidism. Initially, no relevant confounders could be identified.
RESULTS
All requested thyroid parameters were measured with alternative methods, yielding plausible results.
CONCLUSIONS
Biotin is a significant confounder in many immunoassays. Alternative measurement methods or methods of biotin neutralization need to be implemented for certain situations.
背景
利用链霉亲和素与生物素之间相互作用的自动化免疫分析方法被广泛应用。尽管如此，生物素仍然是一个经常被忽视的混杂因素。
方法
我们报告了一例54岁患有进行性多发性硬化症和桥本甲状腺炎的女性患者前来进行随访的病例。在罗氏cobas® 8000模块化分析仪系列上的检测结果提示严重甲亢。最初，未发现相关混杂因素。
结果
所有所需的甲状腺参数均采用替代方法进行测量，结果合理。
结论
生物素在许多免疫分析中是一个重要的混杂因素。在某些情况下，需要采用替代测量方法或生物素中和方法。
Zotero 插件