Department of Urology, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Eur Rev Med Pharmacol Sci. 2019 Feb;23(3):992-1001. doi: 10.26355/eurrev_201902_16986.
MiRNAs have been verified to play a role in the development and progression of prostate cancer (PCa). However, the role of miR-492 in PCa has not been mentioned. We aim to detect the expression of miR-492 in PCa and explore its underlying mechanism.
The relative expression of miR-492 in PCa tissue samples to normal prostate tissues was detected using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The level of miR-492 in PCa-derived cell lines compared with the normal prostate cell line was also measured. Cell counting kit-8 (CCK-8) and colony formation assays were employed to investigate the cell proliferation ability. Transwell assay and wound-healing assays were utilized to explore the cell invasion and migration abilities. Luciferase assay and Western blot were utilized to explore the underlying mechanism of miR-492 in PCa cells.
MiR-492 expressed significantly higher in PCa tissues than that in the normal tissues. Its expression level was also over-expressed in PCa cells compared with that in the normal cells. The up-regulation of miR-492 promoted the growth, invasion, and migration of the cells, while down-regulation had the opposite effects. SOCS2 was identified as a potential target for miR-492 in PCa. Silencing of SOCS2 could neutralize the inhibitory function of miR-492 inhibitor in PCa cells.
This study demonstrated that miR-492 was over-expressed in PCa and exerted tumor-promoting function in PCa cells via repressing SOCS2 expression. This might provide a new sight for future accurate therapy for PCa.
miRNAs 已被证实参与前列腺癌（PCa）的发生发展。然而，miR-492 在 PCa 中的作用尚未提及。本研究旨在检测 miR-492 在 PCa 中的表达，并探讨其潜在机制。
采用实时定量聚合酶链反应（qRT-PCR）检测 PCa 组织样本中 miR-492 的相对表达，并与正常前列腺组织进行比较。还测量了 PCa 衍生细胞系与正常前列腺细胞系中 miR-492 的水平。使用细胞计数试剂盒-8（CCK-8）和集落形成实验来研究细胞增殖能力。采用 Transwell 实验和划痕愈合实验来研究细胞侵袭和迁移能力。通过荧光素酶报告基因实验和 Western blot 实验来探讨 miR-492 在 PCa 细胞中的潜在机制。
miR-492 在 PCa 组织中的表达明显高于正常组织。与正常细胞相比，其在 PCa 细胞中的表达水平也上调。miR-492 的上调促进了细胞的生长、侵袭和迁移，而下调则产生相反的效果。SOCS2 被鉴定为 miR-492 在 PCa 中的潜在靶标。沉默 SOCS2 可以中和 miR-492 抑制剂在 PCa 细胞中的抑制功能。
本研究表明，miR-492 在 PCa 中高表达，并通过抑制 SOCS2 的表达在 PCa 细胞中发挥促肿瘤作用。这可能为未来 PCa 的精准治疗提供新的思路。
