KIRARI Dental Clinic, Oyama, Tochigi, 323-0820, Japan.
Sakai City Medical Center, Sakai, Osaka, 593-8304, Japan.
Chem Biol Interact. 2019 Apr 1;302:143-148. doi: 10.1016/j.cbi.2019.02.006. Epub 2019 Feb 16.
N-(3-oxododecanoyl)-l-homoserine-lactone (ODHL), a quorum sensing molecule, affects intracellular Zn concentration ([Zn]i) and cellular levels of nonprotein thiols ([NPT]i) of rat thymic lymphocytes, both of which are assumed to affect cell vulnerability to oxidative stress. Therefore, it is interesting to examine the effects of ODHL on the cells under oxidative stress. ODHL augmented the cytotoxicity of HO, but not calcium ionophore A23187. ODHL potentiated the HO-induced elevation of [Zn]i, wherein, it greatly attenuated the HO-induced increase in intracellular Ca concentration. ODHL did not affect [NPT]i in the presence of HO. Therefore, we conclude that the elevation of [Zn]i is involved in the ODHL-induced potentiation of HO cytotoxicity. Our findings suggest that ODHL modifies cell vulnerability to oxidative stress in host cells.
N-(3-氧代十二烷酰基)-L-同型半胱氨酸内酯（ODHL），一种群体感应分子，影响大鼠胸腺淋巴细胞的细胞内锌浓度([Zn]i)和非蛋白巯基([NPT]i)水平，这两者都被认为会影响细胞对氧化应激的脆弱性。因此，研究 ODHL 在氧化应激下对细胞的影响很有趣。ODHL 增强了 HO 的细胞毒性，但不能增强钙离子载体 A23187 的毒性。ODHL 增强了 HO 诱导的[Zn]i 升高，其中，它大大减弱了 HO 诱导的细胞内 Ca 浓度增加。ODHL 不影响 HO 存在时的[NPT]i。因此，我们得出结论，[Zn]i 的升高参与了 ODHL 诱导的 HO 细胞毒性增强。我们的研究结果表明，ODHL 改变了宿主细胞对氧化应激的脆弱性。
