尼罗罗非鱼（Oreochromis niloticus）中参与免疫反应细菌感染的半乳糖凝集素-2的特性。
Characterization of Galectin-2 from Nile tilapia (Oreochromis niloticus) involved in the immune response to bacterial infection.
机构信息
College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China.
College of Fishery, Guangdong Ocean University, Zhanjiang, 524088, China; Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
出版信息
Fish Shellfish Immunol. 2019 Apr;87:737-743. doi: 10.1016/j.fsi.2019.02.026. Epub 2019 Feb 16.
galectin-2 plays important roles in innate and adaptive immunity. In this study, galectin-2 (OnGal-2) was identified from Nile tilapia (Oreochromis niloticus). Its tissue distribution and expression patterns following bacterial infection were also investigated. OnGal-2 is widely distributed in various tissues of healthy tilapia. After Streptococcus agalactiae challenge, OnGal-2 expressions were significantly up-regulated in all tested tissues. Meanwhile, the recombinant OnGal-2 (rOnGal-2) protein showed strong agglutinating activities against both Gram-negative bacteria and Gram-positive bacteria. Moreover, rOnGal-2 could promote phagocytosis of macrophages. Taken together, the present study indicated that OnGal-2 might play roles in the immune responses of Nile tilapia against bacterial pathogens.
半乳糖凝集素-2 在先天免疫和适应性免疫中发挥着重要作用。本研究从尼罗罗非鱼（Oreochromis niloticus）中鉴定出半乳糖凝集素-2（OnGal-2）。还研究了其在细菌感染后的组织分布和表达模式。OnGal-2 在健康罗非鱼的各种组织中广泛分布。在受到无乳链球菌（Streptococcus agalactiae）的刺激后，所有检测的组织中 OnGal-2 的表达均显著上调。同时，重组 OnGal-2（rOnGal-2）蛋白对革兰氏阴性菌和革兰氏阳性菌均具有强烈的凝集活性。此外，rOnGal-2 可以促进巨噬细胞的吞噬作用。综上所述，本研究表明 OnGal-2 可能在尼罗罗非鱼抵抗细菌病原体的免疫反应中发挥作用。
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