Lehman Jeffrey S, Igarashi Suzu, Oudemans Peter V
Department of Life and Earth Sciences, Otterbein College, 155 West Main Street, Westerville, OH 43081.
Rutgers University, Blueberry and Cranberry Research and Extension Center, Lake Oswego Rd., Chatsworth, NJ 08019.
Plant Dis. 2007 Jul;91(7):852-856. doi: 10.1094/PDIS-91-7-0852.
Monilinia vaccinii-corymbosi, the causal agent of mummy berry disease, infects blueberry flowers via the gynoecial pathway. To describe the expression of host resistance in highbush blueberry (Vaccinium corymbosum), fungal growth in the styles and colonization of the locules were compared among five blueberry cultivars in a series of controlled greenhouse experiments. Styles were harvested 1 and 4 days postinoculation, and the length colonized by hyphae was determined using fluorescence microscopy. At 8 weeks after inoculation, fruit were harvested and scored for the presence of hyphae in the locules. The infection frequency of styles ranged from 0.33 to 0.71, and only cv. Weymouth had significantly lower infection frequency than the other cultivars. The mean length of the colonized portion of the stylar canal ranged from 0.126 to 0.434 mm after 1 day and 1.62 to 3.59 mm after 4 days. Hyphae in the styles of cv. Weymouth exhibited the least growth, whereas hyphae in the styles of cultivars Jersey and Rancocas were significantly longer. The distance of style penetrated for cultivars Bluecrop and Coville was intermediate. The mean disease incidence of locules differed significantly. Values for cultivars Weymouth and Jersey were the smallest (0.038 and 0.039) and largest (0.249 and 0.236), respectively. The results demonstrate that a component of resistance to infection by M. vaccinii-corymbosi is expressed during growth in the gynoecial pathway.
僵果病的病原菌——蓝莓链核盘菌，通过雌蕊途径侵染蓝莓花。为了描述高丛蓝莓（越橘）中宿主抗性的表达情况，在一系列可控的温室实验中，比较了五个蓝莓品种花柱中的真菌生长情况以及子房的定殖情况。接种后1天和4天收获花柱，使用荧光显微镜确定菌丝定殖的长度。接种8周后，收获果实并对子房内菌丝的存在情况进行评分。花柱的感染频率在0.33至0.71之间，只有品种韦茅斯的感染频率显著低于其他品种。花柱管定殖部分的平均长度在接种1天后为0.126至0.434毫米，接种4天后为1.62至3.59毫米。韦茅斯品种花柱中的菌丝生长最少，而泽西和兰科卡斯品种花柱中的菌丝明显更长。蓝crop和科维尔品种花柱被穿透的距离居中。子房的平均发病率差异显著。韦茅斯和泽西品种的值分别最小（0.038和0.039）和最大（0.249和0.236）。结果表明，对蓝莓链核盘菌感染的抗性成分在雌蕊途径生长过程中表达。
