Orita Hiraku, Sakai Jun-Ichi, Kubota Kenji, Okuda Mitsuru, Tanaka Yuko, Hanada Kaoru, Imamura Yukihisa, Nishiguchi Masamichi, Karasev Alexander V, Miyata Shin-Ichi, Iwanami Toru
National Agricultural Research Center for Kyushu Okinawa Region, Suya 2421, Nishigoshi, Kumamoto 861-1192, Japan.
Miyazaki General Agricultural Research Station, Sadohara, Miyazaki 880-0212, Japan.
Plant Dis. 2007 Dec;91(12):1574-1578. doi: 10.1094/PDIS-91-12-1574.
A new tobamo-like virus was isolated from a greenhouse-grown cucumber that showed severe mosaic distortion on leaves and fruit, in the southern part of Japan. The virus was tentatively designated Cucumber mottle virus (CuMoV) and further characterized. The size and antigenicity of the coat protein (CP) and the complete sequence of the genome were compared with those of the known cucurbit-infecting tobamoviruses: the W and SH strains of Cucumber green mottle mosaic virus (CGMMV), the C and Y strains of Kyuri green mottle mosaic virus (KGMMV), Cucumber fruit mottle mosaic virus (CFMMV), and Zucchini green mottle mosaic virus (ZGMMV). The CP of CuMoV migrated more slowly than those of CGMMV-W and -SH and KGMMV-C and -Y in sodium dodecyl sulfate polyacrylamide gel electrophoresis. In Western blot analysis, the CP of CuMoV cross-reacted weakly with antisera against CGMMV-W and did not react with antisera against KGMMV-Y. The overall nucleotide sequence of CuMoV had 62.5 to 63.5% identity with those of CGMMV-W, -SH, KGMMV-Y, CFMMV, and ZGMMV. The genome organization was characteristic of tobamoviruses, encoding a 131-kb protein, a 188-kb protein, a movement protein (MP), and CP in 5' to 3' order. In the phylogenetic analyses of the CP, CuMoV was placed in a separate lineage from CGMMV-W, -SH, KGMMV-C, -Y, CFMMV, and ZGMMV. The results indicate that CuMoV is a distinct tobamovirus species which represents a third sub-subgroup in the cucurbit-infecting tobamoviruses.
在日本南部，从一个温室种植的黄瓜中分离出一种新的烟草花叶病毒样病毒，该黄瓜叶片和果实出现严重花叶变形症状。该病毒被暂定命名为黄瓜斑驳病毒（CuMoV）并进行了进一步鉴定。将其外壳蛋白（CP）的大小、抗原性以及基因组的完整序列与已知感染葫芦科作物的烟草花叶病毒进行了比较：黄瓜绿斑驳花叶病毒（CGMMV）的W和SH株系、京绿斑驳花叶病毒（KGMMV）的C和Y株系、黄瓜果实斑驳花叶病毒（CFMMV）以及西葫芦绿斑驳花叶病毒（ZGMMV）。在十二烷基硫酸钠聚丙烯酰胺凝胶电泳中，CuMoV的CP迁移速度比CGMMV-W和-SH以及KGMMV-C和-Y的CP迁移速度慢。在蛋白质免疫印迹分析中，CuMoV的CP与抗CGMMV-W抗血清发生弱交叉反应，与抗KGMMV-Y抗血清不发生反应。CuMoV的总体核苷酸序列与CGMMV-W、-SH、KGMMV-Y、CFMMV和ZGMMV的核苷酸序列具有62.5%至63.5%的同一性。基因组结构具有烟草花叶病毒的特征，按5'至3'顺序编码一个131-kb蛋白、一个188-kb蛋白、一个运动蛋白（MP）和CP。在CP的系统发育分析中，CuMoV与CGMMV-W、-SH、KGMMV-C、-Y、CFMMV和ZGMMV处于不同的谱系。结果表明，CuMoV是一种独特的烟草花叶病毒物种，代表了感染葫芦科作物的烟草花叶病毒中的第三个亚亚组。
