Gadomska Grażyna, Ziołkowska Katarzyna, Boinska Joanna, Filipiak Jan, Rość Danuta
Department of Hematology and Malignant Diseases of Hematopoietic System, Faculty of Medicine, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-168 Bydgoszcz, Poland.
Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Collegium Medicum in Bydgoszcz, 85-094 Bydgoszcz, Poland.
Medicina (Kaunas). 2019 Feb 16;55(2):54. doi: 10.3390/medicina55020054.
Recent studies suggest that a vascular endothelial growth factor (VEGF-A) may be involved in the thrombotic process by stimulating the expression of tissue factor in vascular endothelial cells. Tissue factor (TF) can also stimulate the transcription of the gene encoding VEGF-A. The relationship between coagulation and angiogenesis in myeloproliferative neoplasms is not fully understood. The aim of this study was to evaluate the concentration of TF in relation to VEGF-A in the blood of patients with essential thrombocythemia (ET). The study group consisted of 130, newly diagnosed patients with ET (mean age 61 years). The control group consisted of 35 healthy volunteers (mean age 51 years). Concentrations of VEGF-A, TF, and tissue factor pathway inhibitor (TFPI) were analysed using immunoenzymatic methods. TF and TFPI activities were performed using chromogenic assays. The median concentration of TF Ag was 3-fold higher and the TF activity was more than 15-fold higher in ET patients than in normal individuals. There were no statistically significant differences in the TFPI concentration and activity between groups. VEGF-A was significantly increased in patients with ET ( < 0.000001). Analysis of correlations revealed a positive correlation between VEGF-A and TF Ag as well as a positive correlation between VEGF-A and TFPI activity. The simultaneous increase of TF concentration and activity, VEGF-A in the blood of patients with ET, as well as a positive correlation between the concentration of TF and VEGF-A demonstrates the coexistence of TF-dependent coagulation and activation of angiogenesis.
近期研究表明，血管内皮生长因子（VEGF - A）可能通过刺激血管内皮细胞中组织因子的表达而参与血栓形成过程。组织因子（TF）也可刺激编码VEGF - A的基因转录。骨髓增殖性肿瘤中凝血与血管生成之间的关系尚未完全明确。本研究旨在评估原发性血小板增多症（ET）患者血液中TF与VEGF - A相关的浓度。研究组由130例新诊断的ET患者（平均年龄61岁）组成。对照组由35名健康志愿者（平均年龄51岁）组成。采用免疫酶法分析VEGF - A、TF和组织因子途径抑制剂（TFPI）的浓度。使用显色法检测TF和TFPI活性。ET患者中TF Ag的中位浓度比正常个体高3倍，TF活性比正常个体高15倍以上。两组之间TFPI浓度和活性无统计学显著差异。ET患者的VEGF - A显著升高（<0.000001）。相关性分析显示VEGF - A与TF Ag之间呈正相关，VEGF - A与TFPI活性之间也呈正相关。ET患者血液中TF浓度和活性、VEGF - A同时升高，以及TF与VEGF - A浓度之间的正相关表明TF依赖性凝血与血管生成激活并存。
