预测大肠杆菌16S和23S rRNA上形成16S - 23S rRNA复合物的识别位点。

Prediction of the recognition sites on 16S and 23S rRNAs from E. coli for the formation of 16S-23S rRNA complex.

作者信息

Thanaraj T A, Kolaskar A S, Pandit M W

机构信息

Centre for Cellular and Molecular Biology, Hyderabad, India.

出版信息

J Biomol Struct Dyn. 1988 Dec;6(3):587-92. doi: 10.1080/07391102.1988.10506509.

DOI:10.1080/07391102.1988.10506509

PMID:3078239

Abstract

Interactions between RNA molecules have been postulated to play an important role in the assembly of ribosomes. Using the sequence analysis and the search of continuous complementary regions on 16S rRNA and 23S rRNA, the recognition sites involved in the formation of ribosome of E. coli are postulated. The number of postulated sites was narrowed down by taking available experimental data. The suggestive evidence for correct postulation is obtained from sequence comparison studies of 16S and 23S rRNAs from various species. The sites 891-899 and 1195-1203 on 16S rRNA along with the corresponding complementary sites 1904-1912 and 760-768 on 23S rRNA are predicted to be the most probable candidates for the sites of recognition between 16S and 23S rRNAs. The possibility of the involvement of the additional site 630-638 on 16S rRNA with its complementary site 2031-2039 on 23S rRNA cannot be ruled out.

摘要

据推测，RNA分子之间的相互作用在核糖体组装过程中发挥着重要作用。通过对16S rRNA和23S rRNA进行序列分析并搜索连续互补区域，推测出了大肠杆菌核糖体形成过程中涉及的识别位点。利用现有的实验数据，推测出的位点数量有所减少。从对不同物种的16S和23S rRNA进行序列比较研究中获得了支持正确推测的暗示性证据。预计16S rRNA上的891 - 899位点和1195 - 1203位点以及23S rRNA上相应的互补位点1904 - 1912和760 - 768是16S和23S rRNA之间最有可能的识别位点。不能排除16S rRNA上的额外位点630 - 638及其在23S rRNA上的互补位点2031 - 2039参与其中的可能性。