Xing Ronglian, Ma Weiwei, Shao Yiwen, Cao Xuebin, Chen Lihong, Jiang Aili
College of Life Sciences, Yantai University, Yantai, China.
National Algae and Sea Cucumber Project Technology Research Centre, Shandong Oriental Ocean Sci-Tech Co., LTD, Yantai, China.
PeerJ. 2019 Feb 14;7:e6468. doi: 10.7717/peerj.6468. eCollection 2019.
, dominant filamentous green algae, can be harmful to sea cucumber growth in aquaculture ponds of China. In order to understand the environmental factors affecting the growth of in sea cucumber aquaculture ecosystems, a combination of field investigations and laboratory experiments were conducted. Field surveys over one year revealed that survived in sea cucumber aquaculture ponds in salinities ranging from 24.3 ± 0.01‰ to 32.0 ± 0.02‰ and a pH range of 7.5 ± 0.02-8.6 ± 0.04. The high salinity and pH during the period of low biomass from January to May lay the foundation for its rapid growth in the following months of June to October. Many factors interact in the field environment, thus, laboratory experiments were conducted to determine the isolated effects of pH and salinity on growth. In laboratory experiments, samples were incubated under different salinity and pH conditions at 25 °C, with a light intensity of 108 μmol photon·m·s, and a photoperiod of 12 L:12 D. Results showed that salinity and pH significantly affect the growth and / (quantum yield of photosynthesis) of ( < 0.01). grew the longest at a salinity of 34‰ and a pH of 8.0. At 34‰ salinity, grew to 26.44 ± 5.89 cm in 16 days. At a pH of 8.0, grew to 67.96 ± 4.45 cm in 32 days. / was 0.635 ± 0.002 at a salinity of 32‰, and 0.550 ± 0.006 to 0.660± 0.001 at pH 7.0 to 8.5. Based on these results, we conclude that can bloom in sea cucumber ponds due to the high salinity and pH of coastal sea waters, which promote growth and maintain the photosynthetic activity of .
优势丝状绿藻对中国海参养殖池塘中海参的生长可能有害。为了解海参养殖生态系统中影响该绿藻生长的环境因素，开展了实地调查与实验室实验相结合的研究。为期一年的实地调查显示，该绿藻在海参养殖池塘中生存的盐度范围为24.3±0.01‰至32.0±0.02‰，pH值范围为7.5±0.02至8.6±0.04。1月至5月该绿藻生物量较低期间的高盐度和pH值为其在随后6月至10月的快速生长奠定了基础。野外环境中多种因素相互作用，因此进行了实验室实验以确定pH值和盐度对该绿藻生长的单独影响。在实验室实验中，样本在25℃、光照强度为108μmol光子·m·s、光周期为12小时光照:12小时黑暗的不同盐度和pH值条件下培养。结果表明，盐度和pH值显著影响该绿藻的生长及光合量子产量（<0.01）。在盐度为34‰、pH值为8.0时，该绿藻生长最长。在34‰盐度下，16天内该绿藻长至26.44±5.89厘米。在pH值为8.0时，32天内该绿藻长至67.96±4.45厘米。在32‰盐度下，光合量子产量为0.635±0.002，在pH值7.0至8.5时为0.550±0.006至0.660±0.001之间。基于这些结果，我们得出结论，由于沿海水域的高盐度和pH值促进了该绿藻的生长并维持其光合活性，所以它能在海参池塘中大量繁殖。
