Zheng Fei, Yu Huimin, Lu Jingjing
Department of Gynecology, Ningbo No. 2 Hospital, Ningbo, Zhejiang, China.
J Cell Biochem. 2019 Jul;120(7):11859-11866. doi: 10.1002/jcb.28466. Epub 2019 Feb 19.
Mucins (MUCs) have been reported to play a critical role in the tumorigenesis of different cancers. This study was performed to explore the effect of MUC20 in endometrial cancer (EC). A total of 541 patients with EC were examined from The Cancer Genome Atlas. The relationship between MUC20 expression and clinical characteristics was analyzed with the Wilcoxon signed-rank test and logistic regression. The Kaplan-Meier method and the Cox regression model was performed to evaluate the prognosis. Gene set enrichment analysis (GSEA) was conducted. MUC20 high expression was associated with age, histology, positive peritoneal cytology, advanced stage, and lymph node metastasis (P < 0.05). Kaplan-Meier survival showed that patients with MUC20 high expression had a poorer prognosis than those with MUC20 low expression. Furthermore, multivariate analysis showed that MUC20 high expression was an independent prognostic factor for worse overall survival (hazard ratio = 1.93, 95% confidence interval = 1.00-3.74). Moreover, interferon α/γ response, cell-cell adhesion, O-glycan processing, and reactive oxygen species (ROS) pathway were associated with MUC20 high expression. MUC20 high expression may be a potential prognostic molecular factor of poor survival. The interferon α/γ response, cell-cell adhesion, O-glycan processing, and ROS pathway may be the key processes regulated by MUC20 in EC.
据报道，黏蛋白（MUCs）在不同癌症的肿瘤发生中起关键作用。本研究旨在探讨MUC20在子宫内膜癌（EC）中的作用。从癌症基因组图谱中检查了总共541例EC患者。采用Wilcoxon符号秩检验和逻辑回归分析MUC20表达与临床特征之间的关系。采用Kaplan-Meier法和Cox回归模型评估预后。进行基因集富集分析（GSEA）。MUC20高表达与年龄、组织学、腹膜细胞学阳性、晚期和淋巴结转移相关（P<0.05）。Kaplan-Meier生存分析显示，MUC20高表达患者的预后比MUC20低表达患者差。此外，多因素分析表明，MUC20高表达是总体生存较差的独立预后因素（风险比=1.93，95%置信区间=1.00-3.74）。此外，干扰素α/γ反应、细胞间黏附、O-聚糖加工和活性氧（ROS）途径与MUC20高表达相关。MUC20高表达可能是生存不良的潜在预后分子因素。干扰素α/γ反应、细胞间黏附、O-聚糖加工和ROS途径可能是MUC20在EC中调节的关键过程。
