Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia; Department of Biodiversity, The School of Natural Sciences, Far Eastern Federal University, Vladivostok, Russia.
Laboratory of Biotechnology, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690022, Russia.
J Plant Physiol. 2019 Mar-Apr;234-235:133-137. doi: 10.1016/j.jplph.2019.02.002. Epub 2019 Feb 14.
This study examines the effects of p-coumaric (CoA) and caffeic (CaA) acids on stilbene biosynthesis in one-year-old needles of Picea jezoensis (Lindl. et Gord.) Fisch ex Carr. under control conditions and after ultraviolet (UV-C) irradiation. HPLC analysis revealed that while UV-C irradiation slightly affected the total stilbene content, CoA and CaA feeding increased the total content of stilbenes by 1.2-1.3-fold. UV-C treatment combined with CoA-feeding of the P. jezoensis cuttings exerted the most pronounced stimulatory effect on stilbene accumulation leading to the total stilbene content of 9.18 mg/g of dry weight or DW. This increase correlated with the elevated transcription of the stilbene synthase PjSTS1a and PjSTS1b genes. UV-C treatment in combination with CaA feeding of P. jezoensis did not considerably influence stilbene content. These results revealed a positive effect of UV-C radiation and phenolic precursors on the content of stilbenes in spruce needles.
本研究考察了对香豆酸（CoA）和咖啡酸（CaA）对云杉（Picea jezoensis（Lindl. et Gord.）Fisch ex Carr.）一年生针叶在对照条件和紫外线（UV-C）辐射后的芪类生物合成的影响。HPLC 分析表明，虽然 UV-C 辐射对总芪含量略有影响，但 CoA 和 CaA 的喂养使芪类的总含量增加了 1.2-1.3 倍。UV-C 处理结合 CoA 喂养云杉插条对芪类物质的积累产生了最显著的刺激作用，导致总芪含量达到 9.18mg/g 干重（DW）。这种增加与芪合酶 PjSTS1a 和 PjSTS1b 基因的转录升高有关。UV-C 处理结合 CaA 喂养对云杉芪类物质含量没有显著影响。这些结果表明，UV-C 辐射和酚类前体对云杉针叶芪类物质含量有积极影响。
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