Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran.
Andrology. 2019 May;7(3):350-356. doi: 10.1111/andr.12596. Epub 2019 Feb 20.
Reproductive toxicity of lipopolysaccharide (LPS) on spermatozoa is well established.
The aim of the present study was to show the potential benefits of alpha lipoic acid (ALA) as a strong antioxidant in alleviating the reproductive toxicity of LPS.
Sperm cells and cumulus-oocyte complexes (COCs) were collected from healthy NMRI mice (body weights ranged from 25 to 35 g, 100 females and 200 males). Sperm cells were treated with varying doses of ALA (0.01, 0.02, and 0.04 mm) and 0.01 μg/mL of LPS for 4 h. The quality of spermatozoa (ROS production, DNA fragmentation, and spontaneous acrosome reaction), sperm fertilizability, and the consequent developmental competence of oocytes inseminated with ALA/LPS-treated spermatozoa were recorded.
The results showed that 0.04 mm of ALA abrogated LPS-reduced sperm motility, viability, ROS production, spontaneous acrosome reaction, fertilizability, and developmental competence. In addition, 0.04 mm ALA significantly reverted the negative effects of LPS on inner cell mass (ICM) cell counts, total cell number (TCM), and ratio between ICM and TCM.
Our data showed that ALA significantly could abrogate the negative effects of LPS on sperm quality and oocyte developmental competence. Therefore, ALA had the capacity for protecting sperm cells from LPS-induced damage and ensured fertilization and developmental competency.
These in vitro findings suggested a therapeutic role for ALA in reducing the negative effects of LPS on spermatozoa and early embryonic development.
脂多糖 (LPS) 对精子的生殖毒性已得到充分证实。
本研究旨在展示α-硫辛酸 (ALA) 作为一种强大的抗氧化剂在缓解 LPS 生殖毒性方面的潜在益处。
从健康的 NMRI 小鼠（体重范围为 25 至 35 克，100 只雌性和 200 只雄性）中收集精子细胞和卵丘-卵母细胞复合物 (COC)。将精子细胞用不同剂量的 ALA（0.01、0.02 和 0.04 mM）和 0.01 μg/mL LPS 处理 4 小时。记录精子质量（ROS 产生、DNA 碎片化和自发顶体反应）、精子受精能力以及用 ALA/LPS 处理的精子授精后的卵母细胞随后的发育能力。
结果表明，0.04 mM 的 ALA 阻断了 LPS 降低的精子活力、活力、ROS 产生、自发顶体反应、受精能力和发育能力。此外，0.04 mM ALA 显著逆转了 LPS 对内细胞团 (ICM) 细胞计数、总细胞数 (TCM) 和 ICM 与 TCM 之间的比例的负面影响。
我们的数据表明，ALA 可显著减轻 LPS 对精子质量和卵母细胞发育能力的负面影响。因此，ALA 具有保护精子细胞免受 LPS 诱导损伤的能力，并确保受精和发育能力。
这些体外研究结果表明，ALA 在降低 LPS 对精子和早期胚胎发育的负面影响方面具有治疗作用。
