Increase of germ cell nuclear factor expression in globozoospermic Gopc knockout mice.

BACKGROUND

Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) is a Golgi protein that plays a role in vesicular transport and intracellular protein trafficking and degradation. Mice deficient in GOPC protein have globozoospermia and are infertile. The germ cell nuclear factor (GCNF) is a member of the nuclear receptor superfamily which is expressed in male germ cells, from spermatocytes and spermatids, both in the nucleus and the acrosomal region. It is not known if its expression could be altered in Gopc mice with defective acrosomes.

OBJECTIVES

The aim of the present work was to analyze the distribution of GCNF protein in spermatids of Gopc knockout mice.

MATERIALS AND METHODS

We have analyzed the expression and distribution during spermatogenesis of GCNF and its deregulation in Gopc mutant mice by RT-qPCR, Western blot, immunohistochemistry and immunogold.

RESULTS

Germ cell nuclear factor was localized in the nucleus of all the cell types in the seminiferous tubules. Despite being a nuclear protein, it was also located in the acrosome and in the manchette of elongating spermatids. We have found that in the absence of GOPC, the expression of GCNF was increased in the nucleus of spermatocytes, mainly in leptotene, and in the nucleus and the manchette during the spermatid elongation.

DISCUSSION AND CONCLUSION

Gopc mice have defective acrosome and manchette. The manchette is involved in the transport of proteins through the cytoplasm and the nucleus. Considering that the GCNF protein is normally transported to the acrosome and the nucleus, it can be thought that transport deficiencies in Gopc mice are responsible for the increased expression of this protein.