Feng Shu, Wang Junfu, Xu Xiaoqun, Chen Xuemei, Luan Junwen, Su Qinghong, Luan Meng, Wang Huali, Zhao Changsheng
Dept. of Clinical Laboratory, Shandong Provincial Hospital, Jinan, Shandong, China.
Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong, China.
Iran J Public Health. 2018 Dec;47(12):1874-1882.
Hypopharyngeal carcinoma is one of the most common types of head and neck tumors. Suppressers of cytokine signalling (SOCS) family members are key regulators of cytokine homeostasis, they play important roles in the process of cell proliferation, differentiation, maturation and apoptosis, and participate in the occurrence and development of tumor. The abnormal activation of NF-ϰB is an important feature of the tumor. The aim of this study was to investigate the relationships among SOCS, NF-ϰB p65 and hypopharyngeal carcinoma development.C.
We included 72 hypopharyngeal cancer patients and 9 swallow cyst patients. The patients were recruited at The Second Hospital of Shandong University (Jinan, China) between 2014 and 2016. The mRNA and protein expression levels of SOCS-1, SOCS-3 and NF-ϰB p65 in hypopharyngeal carcinoma tissues, para-cancerous tissues and control tissues were detected by RT-PCR and Western blot analysis, respectively.
Hypopharyngeal carcinoma tissues had lower level expression of SOCS-1 and SOCS-3 than pericarcinoma tissues, but there was no significant difference, while cancer tissues had significantly higher level expression of NF-ϰB p65 than that of pericarcinoma tissues (0.412±0.266, 0.281±0.231, t=2.969, =0.004). The early stage patients had striking higher level expression of SOCS-1 and SOCS-3 than that in advanced stages (F=16.202, <0.001; F=52.295, <0.001), while the expression of NF-ϰB p65 in early stages had lower level than that in advanced stages (F=3.383, =0.04).
SOCS-1, SOCS-3 may be protective factors while NF-ϰB p65 could be a harmful factor in hypopharyngeal carcinoma.
下咽癌是头颈部肿瘤最常见的类型之一。细胞因子信号转导抑制因子（SOCS）家族成员是细胞因子稳态的关键调节因子，在细胞增殖、分化、成熟及凋亡过程中发挥重要作用，并参与肿瘤的发生发展。核因子-κB（NF-κB）的异常激活是肿瘤的一个重要特征。本研究旨在探讨SOCS、NF-κB p65与下咽癌发生发展之间的关系。
我们纳入了72例下咽癌患者和9例下咽囊肿患者。这些患者于2014年至2016年在山东大学第二医院（中国济南）招募。分别通过逆转录-聚合酶链反应（RT-PCR）和蛋白质免疫印迹分析检测下咽癌组织、癌旁组织及对照组织中SOCS-1、SOCS-3和NF-κB p65的mRNA和蛋白表达水平。
下咽癌组织中SOCS-1和SOCS-3的表达水平低于癌旁组织，但差异无统计学意义，而癌组织中NF-κB p65的表达水平显著高于癌旁组织（0.412±0.266，0.281±0.231，t = 2.969，P = 0.004）。早期患者SOCS-1和SOCS-3的表达水平显著高于晚期患者（F = 16.202，P < 0.001；F = 52.295，P < 0.001），而早期NF-κB p65的表达水平低于晚期（F = 3.383，P = 0.04）。
在下咽癌中，SOCS-1、SOCS-3可能是保护因素，而NF-κB p65可能是有害因素。
