Matveenko Andrew G, Drozdova Polina B, Moskalenko Svetlana E, Tarasov Oleg V, Zhouravleva Galina A
Department of Genetics and Biotechnology, Saint Petersburg State University, St. Petersburg 199034, Russia.
St. Petersburg Branch, Vavilov Institute of General Genetics, Russian Academy of Sciences, St. Petersburg 199034, Russia.
Data Brief. 2019 Jan 19;23:103694. doi: 10.1016/j.dib.2019.01.042. eCollection 2019 Apr.
Termination of translation in eukaryotes is governed by two release factors encoded by the and genes in . Previously, a set of mutations in these genes had been obtained. However, the exact sequence change associated with one mutation, , was not identified by Sanger sequencing of the region. Presented here are whole-genome sequencing data for the strain, data on copy number variation in its genome along with supporting pulse-field gel electrophoresis experiment data, and the list of single-nucleotide variations that differentiate this strain and its wild-type ancestor. One substitution upstream the gene was located in a sequence corresponding to the Abf1-binding site. Data obtained from the introduction of this variation from strain into a different wild-type strain, specifically, detection of a nonsense-suppressor phenotype accompanied by a decrease in the Sup35 protein level, are also presented in this article.
真核生物中翻译的终止由酵母中SUP45和SUP35基因编码的两个释放因子控制。此前，已获得了这些基因中的一组突变。然而，通过对SUP35区域的桑格测序未鉴定出与一个名为sup35-4的突变相关的确切序列变化。本文展示了sup35-4菌株的全基因组测序数据、其基因组中拷贝数变异的数据以及支持脉冲场凝胶电泳实验的数据，还有区分该菌株与其野生型祖先的单核苷酸变异列表。在SUP35基因上游的一个替换位于对应于Abf1结合位点的序列中。本文还展示了将该变异从sup35-4菌株引入不同野生型菌株后获得的数据，具体而言，检测到无义抑制子表型并伴有Sup35蛋白水平降低。
