Zhang Jing, Miao Gang, Cao Jian-Min, Zhou Hai-Tao, Niu Yan-Long, Zhang Yue, Ren Yi, Bao Xin-Yue, Xing Ya-Wen
Biochemical Engineering College of Beijing Union University, Beijing 100023.
Key Laboratory of Bioactive Substances and Functional Foods, Beijing Union University, Beijing 100191.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2018 May 8;34(5):445-449. doi: 10.12047/j.cjap.5688.2018.101.
To study the effects of aerobic exercise combined with chlorella pyrenoidos of disintegrated cell wall on the lipid metabolism in rats with high-fat diet.
Fifty-five male Wistar rats were subjected to adaptive feeding for 4 days and weight-free swimming training for 3 days, 20 min/d. After eliminating 5 rats that were not suitable for swimming training, the other rats were randomly divided into 5 groups according to their body weight:control group (C group), high fat diet group (H group), high-fat diet + chlorella group(HC group), high fat diet + aerobic exercise group (HM group), high fat diet + chlorella + aerobic exercise group (HMC group), 10 in each group. The HM and HMC group were subjected to 60 min/d swimming training for 6 weeks with non-weight-bearing. Group C were fed regular diet. The other groups were fed with high-fat diet, the rats in group HC and HMC were intragastrically treated with chlorella pyrenoidos of disintegrated cell wall at the dose of 3.9 g/(kg·d), the volume was 5 ml/kg, and the other groups are given equivalent saline. The Lee's index and biochemical indexes of blood and liver were measured after 6 weeks.
Compared with group C, Lee's index, serum levels of free fatty acids(FFA), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-c), liver FFA and interleukin-10 (IL-10) were increased significantly (<0.01), the serum level of high-density lipoprotein cholesterol (HDL-c) was decreased significantly (<0.01) in group H. Compared with group H, Lee's index, serum FFA, IL-6, TNF-α, TC, TG, LDL-c, liver FFA and IL-10 were decreased significantly (<0.05 or <0.01), serum level of HDL-c was increased significantly (<0.05 or <0.01) in group HC, HM and HMC. Compared with group HC and HM, Lee's index, serum FFA, IL-6, TNF-α, TC, TG, LDL-c, liver FFA and IL-10 were decreased significantly (<0.05), serum level of HDL-c was increased significantly (<0.05) in group HMC.
Aerobic exercise and chlorella pyrenoidos of disintegrated cell wall can improve lipid metabolism in rats with high-fat diet and reduce the lipid toxicity caused by obesity. Joint intervention is more effective than single intervention.
研究有氧运动联合破壁小球藻对高脂饮食大鼠脂质代谢的影响。
55只雄性Wistar大鼠先进行4天适应性喂养，然后进行3天无负重游泳训练，每天20分钟。剔除5只不适合游泳训练的大鼠后，将其余大鼠按体重随机分为5组：对照组（C组）、高脂饮食组（H组）、高脂饮食+小球藻组（HC组）、高脂饮食+有氧运动组（HM组）、高脂饮食+小球藻+有氧运动组（HMC组），每组10只。HM组和HMC组进行6周无负重游泳训练，每天60分钟。C组喂常规饲料。其他组喂高脂饲料，HC组和HMC组大鼠按3.9 g/(kg·d)剂量灌胃给予破壁小球藻，体积为5 ml/kg，其他组给予等量生理盐水。6周后测量Lee's指数及血液和肝脏生化指标。
与C组相比，H组Lee's指数、血清游离脂肪酸（FFA）、白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-c）、肝脏FFA和白细胞介素-10（IL-10）显著升高（<0.01），血清高密度脂蛋白胆固醇（HDL-c）水平显著降低（<0.01）。与H组相比，HC组、HM组和HMC组Lee's指数及血清FFA、IL-6、TNF-α、TC、TG、LDL-c、肝脏FFA和IL-10显著降低（<0.05或<0.01），血清HDL-c水平显著升高（<0.05或<0.01）。与HC组和HM组相比，HMC组Lee's指数及血清FFA、IL-6、TNF-α、TC、TG、LDL-c、肝脏FFA和IL-10显著降低（<0.05），血清HDL-c水平显著升高（<0.05）。
有氧运动和破壁小球藻可改善高脂饮食大鼠的脂质代谢，降低肥胖引起的脂质毒性。联合干预比单一干预更有效。
