College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application , Nanjing Agricultural University , Nanjing 210095 , P. R. China.
Key Laboratory of Integrated Management of Crop Diseases and Pests , Ministry of Education , Nanjing 210095 , P. R. China.
J Agric Food Chem. 2019 Mar 13;67(10):2811-2817. doi: 10.1021/acs.jafc.8b04996. Epub 2019 Feb 27.
Five alkylitaconic acid (AA) derivatives, including two novel compounds, epideoxysporothric acid (2) and sporochartine F (5), and three known compounds, deoxysporothric acid (1), deoxyisosporothric acid (3), and 1-undecen-2,3-dicarboxylic acid (4), were obtained from the fermentation culture of the endophytic fungus Nodulisporium sp. A21. The auxin herbicidal activities of compounds 1-4 against weed seeds were investigated under laboratory conditions. In general, the tested compounds displayed radicle growth promoting activity at low doses and inhibitory activity at higher doses. Compounds 1 and 2 could significantly inhibit the radicle growth of dicotyledon weeds, Eclipta prostrata and Veronica persica, at a concentration range from 50 to 200 μg mL, while 3 notably stimulated radicle growth at the same concentration range. The results suggested that these AA derivatives have the potential to be used as the lead scaffold for novel auxin herbicide development. In addition, the biosynthetic pathways of 1-4 were deduced based on C labeling experiment.
从内生真菌 Nodulisporium sp. A21 的发酵培养物中获得了 5 种烷基衣康酸（AA）衍生物，包括两种新化合物表脱枝孢酸（2）和孢子查汀 F（5），以及三种已知化合物脱枝孢酸（1）、去氧异脱枝孢酸（3）和 1-十一烯-2,3-二羧酸（4）。在实验室条件下，研究了化合物 1-4 对杂草种子的生长素除草活性。一般来说，测试化合物在低剂量下表现出促进主根生长的活性，在高剂量下表现出抑制活性。化合物 1 和 2 可在 50-200μg/mL 的浓度范围内显著抑制双子叶杂草繁缕和婆婆纳的主根生长，而 3 在相同浓度范围内则明显刺激主根生长。结果表明，这些 AA 衍生物有可能被用作新型生长素除草剂开发的先导支架。此外，还基于 C 标记实验推导出了 1-4 的生物合成途径。
