Department of Neurosurgery, The First Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang, 150001, People's Republic of China.
Department of Pathology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, People's Republic of China.
Cell Mol Neurobiol. 2019 Apr;39(3):461-470. doi: 10.1007/s10571-019-00662-y. Epub 2019 Feb 21.
Temporal lobe epilepsy (TLE) is associated with neurodegeneration, often leading to hippocampal sclerosis (HS). Type 1 HS, which is characterized by severe neuronal loss and gliosis predominantly in regions CA1 and CA4, is the most common subtype and is associated with the best prognosis according to the ILAE classification system. MiRNAs participate in the biological processes underlying many nervous system diseases, including epilepsy. However, the miRNA expression profile of HS ILAE type 1 is not completely understood. A total of 14 patients were identified as having the ILAE subtype, as determined by NeuN immunohistochemistry (ILAE type 1 = 7; no-HS = 7). Next-generation sequencing and reverse transcription polymerase chain reaction technology were used to validate the dysregulated miRNAs. Bioinformatics analysis of the predicted target genes was conducted using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. In total, 1643 mature miRNAs were detected in this study, along with 5 miRNAs that were upregulated and 2 miRNAs that were downregulated in the type 1 group. Bioinformatics analysis showed that 1545 target genes were predicted using the miRDB and Targetscan databases and that these predicted genes showed enrichment in pathways associated with nucleic acid binding, intracellular and cellular macromolecule metabolic processes, and the PI3K-Akt signaling pathway. This study is the first to report the miRNA expression profile of HS ILAE type 1 compared with those of no-HS. These results provide new insights into the neuronal loss pathology of type 1 HS.
颞叶癫痫（TLE）与神经退行性变有关，常导致海马硬化（HS）。1 型 HS 以 CA1 和 CA4 区域为主的严重神经元丢失和神经胶质增生为特征，是最常见的亚型，根据 ILAE 分类系统，其预后最好。miRNA 参与许多神经系统疾病的生物学过程，包括癫痫。然而，1 型 ILAE 型 HS 的 miRNA 表达谱尚不完全清楚。通过 NeuN 免疫组织化学（ILAE 1 型=7；无 HS=7）确定了总共 14 名患者具有 ILAE 亚型。使用下一代测序和逆转录聚合酶链反应技术验证失调的 miRNA。使用基因本体论和京都基因与基因组百科全书分析对预测的靶基因进行生物信息学分析。本研究共检测到 1643 个成熟 miRNA，其中 5 个 miRNA 在 1 型组中上调，2 个 miRNA 下调。生物信息学分析显示，miRDB 和 Targetscan 数据库预测到了 1545 个靶基因，这些预测基因在与核酸结合、细胞内和细胞大分子代谢过程以及 PI3K-Akt 信号通路相关的途径中富集。这是首次报道 ILAE 1 型 HS 与无 HS 相比的 miRNA 表达谱。这些结果为 1 型 HS 的神经元丢失病理学提供了新的见解。
