Miller C H, Carbonell A R, Peng R, MacKenzie M R, Shifrine M
Am J Vet Res. 1978 Jul;39(7):1191-4.
Reference values for T and B lymphocytes were determined on lymphocytes from canine thymus, spleen, lymph node, bone marrow, and peripheral blood by use of erythrocyte (E) and erythrocyte-antibody-complement (EAC) rosette assays, plus a direct fluorescent technique for assay of surface immunoglobulins. Numbers of T lymphocytes, indicated by E rosette formation with human erythrocytes, ranged from a low of 1% in the thymus to 13% in the peripheral blood, whereas B-lymphocyte numbers ranged from 3% (thymus) to 41% (bone marrow) and from 6% (thymus) to 36% (bone marrow), as indicated by EAC rosette formation or presence of surface immunoglobulins respectively. Stimulation of peripheral blood lymphocytes with either phytohemagglutinin or concanavalin A increased the total number of E-rosetting cells two to threefold, whereas the number of EAC-rosetting cells decreased by half. Further, the percentage of cells bearing Fc receptors increased after phytohemagglutinin stimulation. These results indicate the E rosette technique can be used to identify and to monitor a population of canine T lymphocytes.
通过使用红细胞（E）和红细胞-抗体-补体（EAC）玫瑰花结试验，以及用于检测表面免疫球蛋白的直接荧光技术，测定了犬胸腺、脾脏、淋巴结、骨髓和外周血淋巴细胞中T和B淋巴细胞的参考值。用人红细胞形成E玫瑰花结所显示的T淋巴细胞数量，在胸腺中低至1%，在外周血中为13%；而EAC玫瑰花结形成或表面免疫球蛋白的存在分别显示，B淋巴细胞数量在胸腺中为3%，在骨髓中为41%，在外周血中为6%，在骨髓中为36%。用植物血凝素或刀豆球蛋白A刺激外周血淋巴细胞，可使E玫瑰花结形成细胞的总数增加两到三倍，而EAC玫瑰花结形成细胞的数量减少一半。此外，植物血凝素刺激后，带有Fc受体的细胞百分比增加。这些结果表明，E玫瑰花结技术可用于鉴定和监测犬T淋巴细胞群体。
