Fauci A S
J Clin Invest. 1975 Jul;56(1):98-110. doi: 10.1172/JCI108085.
This study was undertaken to determine the proportions and in vitro immune capacities of lymphocyte populations in the bone marrows of normal humans. Relatively pure mononuclear cell suspensions were obtained from bone marrow aspirates by linear sucrose gradient centrifugations. Simultaneous peripheral blood and bone marrow specimens from each individual were assayed for lymphocyte surface markers and mitogen responsiveness. Maximal possible contamination of bone marrow aspirates by peripheral blood was determined by performing aspirates on individuals who had received 51chromium-labeled autologous erythrocytes. Rhymus-derived (T) lymphocytes, as determined by the sheep red blood cell (E) rosette assay, comprised 8.6-(plus or minus 1.6)% of the total bone marrow lymphocyte pool. Bone marrow-derived (B) lymphocytes, as determined by the presence of a complement receptor, made up 15.4-(plus or minus 1.9)% of the lymphocyte pool whereas 74.6 (plus or minus 2.4)% of mononuclear cells lacked easily detectable surface markers. These findings could not be explained by contamination with peripheral blood lymphocytes since contamination was corrected for in the calculations. Lymphocyte-enriched suspensions of bone marrow cells responded to stimulation with phytohemagglutinin, concanalin A, and particularly pokeweed mitogen. In vitro incubations of bone marrow and peripheral blood lymphocytes with tritiated thymidine followed by determinations of E and erythrocyte antibody complement (EAC) rosettes were performed. Simultaneous rosetteradioautographs demonstrated that the proliferative potential of bone marrow B lymphocytes was greater than peripheral blood B lymphocytes (P less than 0.01). On the other hand, the proliferative potential of bone marrow T lymphocytes was the same as that of peripheral blood T lymphocytes. These findings demonstrate that in addition to containing B lymphocytes the normal bone marrow contains a small fraction of T lymphocytes similar to the mature T lymphocyte pool found in the peripheral blood. These T cells most probably enter the bone marrow parenchyma as part of the normal recirculating lymphocyte pool.
本研究旨在确定正常人类骨髓中淋巴细胞群体的比例及其体外免疫能力。通过线性蔗糖梯度离心从骨髓穿刺物中获得相对纯净的单核细胞悬液。对每个个体的外周血和骨髓标本同时进行淋巴细胞表面标志物和丝裂原反应性检测。通过对接受过51铬标记自体红细胞的个体进行穿刺，确定骨髓穿刺物被外周血污染的最大可能程度。通过绵羊红细胞（E）花环试验确定，胸腺来源的（T）淋巴细胞占骨髓淋巴细胞总数的8.6%（±1.6%）。通过补体受体的存在确定，骨髓来源的（B）淋巴细胞占淋巴细胞总数的15.4%（±1.9%），而74.6%（±2.4%）的单核细胞缺乏易于检测的表面标志物。这些发现无法用外周血淋巴细胞污染来解释，因为在计算中已对污染进行了校正。富含淋巴细胞的骨髓细胞悬液对植物血凝素、刀豆蛋白A，尤其是商陆丝裂原的刺激有反应。进行了用氚标记胸腺嘧啶核苷对骨髓和外周血淋巴细胞进行体外培养，随后测定E和红细胞抗体补体（EAC）花环的实验。同时进行的花环放射自显影表明，骨髓B淋巴细胞的增殖潜力大于外周血B淋巴细胞（P<0.01）。另一方面，骨髓T淋巴细胞的增殖潜力与外周血T淋巴细胞相同。这些发现表明，正常骨髓除了含有B淋巴细胞外，还含有一小部分与外周血中成熟T淋巴细胞池相似的T淋巴细胞。这些T细胞很可能作为正常再循环淋巴细胞池一部分进入骨髓实质。
