PPG Biodiverdade Animal, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
Department of Biochemistry and Molecular Biology, CCNE, Univeridade Federal de Santa Maria, Santa Maria, Brazil.
Insect Mol Biol. 2019 Oct;28(5):616-627. doi: 10.1111/imb.12577. Epub 2019 Mar 12.
A monophyletic group of Drosophila hAT transposable elements, referred to as Herves-like, was characterized and found to be present in 46% of 57 screened Drosophila species. A remarkable characteristic of these elements is the presence of a long array of minisatellite repeats (MnRs) in both subterminal extremities of the elements. The copy number of these minisatellites was highly variable between and within populations. Twenty-three strains of Drosophila willistoni, covering its geographic distribution, were screened for polymorphism in the copy number of 5' MnRs, showing a variation from 7 to 20 repeat copies. These MnRs are well conserved among Drosophila species and probably function as transposase binding sequences, as provided by short subterminal repeats in other hAT elements. Miniature inverted repeat transposable elements were found in 27% of species carrying Herves-like elements. Phylogenetic analysis showed incongruences between transposable elements and species phylogenies, suggesting that at least four horizontal transfer events have occurred.
一类被称为 Herves 样的果蝇 hAT 转座元件的单系群，其特征是在筛选的 57 种果蝇中，有 46%存在这些元件。这些元件的一个显著特点是在元件的两个亚末端都存在长串的微卫星重复序列（MnRs）。这些微卫星在种群之间和种群内部的数量存在高度的可变性。对涵盖其地理分布的 23 个 Drosophila willistoni 品系进行了 5' MnRs 拷贝数的多态性筛选，显示重复拷贝数从 7 到 20 不等。这些 MnRs 在果蝇种间高度保守，可能作为转座酶结合序列发挥作用，正如其他 hAT 元件中的短亚末端重复序列所提供的那样。携带 Herves 样元件的 27%的物种中发现了微型反向重复转座元件。系统发育分析表明，转座元件与物种系统发育之间存在不一致，表明至少发生了 4 次水平转移事件。
