Obasa Adetayo Emmanuel, Ashokkumar Manickam, Neogi Ujjwal, Jacobs Graeme Brendon
1 Division of Medical Virology, Department of Pathology, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
2 Division of Clinical Microbiology, Department of Laboratory Medicine, Karolinska Institute, University of Stockholm, Stockholm, Sweden.
AIDS Res Hum Retroviruses. 2019 Jun;35(6):572-576. doi: 10.1089/AID.2018.0293. Epub 2019 Apr 4.
HIV-1 subtype C (HIV-1C) is responsible for the majority of infections in sub-Saharan Africa. We selected 63 plasma-derived samples and generated long terminal repeats (LTRs) amplicons from people living with HIV in South Africa to identify transcription factor binding sites. NF-κB plays an important role in regulating the viral gene expression from the viral promoter and controlling viral latency. LTR amplicons were sequenced and phylogenetically analyzed. In our data set, we identified F-κB sites ( = 4; 6%) at position II and ( = 1; 1%) at position I among 63 sequences analyzed. The majority of the sequences identified with H-κB at position II ( = 50; 79%) and position I ( = 55; 87%). Forty-nine ( = 49; 78%) sequences were found to exhibit C-κB site. ZA_LTR052 was identified with a single point mutation. We identified all three NF-κB-binding sites in ( = 44; 70%) the viral promoter-enhancer regions in South African patients.
HIV-1 C亚型（HIV-1C）是撒哈拉以南非洲地区大多数感染病例的病原体。我们选取了63份血浆来源样本，从南非的HIV感染者中扩增出长末端重复序列（LTR），以确定转录因子结合位点。NF-κB在调控病毒启动子的病毒基因表达以及控制病毒潜伏方面发挥着重要作用。对LTR扩增子进行测序并进行系统发育分析。在我们的数据集中，在分析的63个序列中，我们在II位鉴定出4个（6%）F-κB位点，在I位鉴定出1个（1%）F-κB位点。在II位和I位，大多数序列鉴定出H-κB位点，分别为50个（79%）和55个（87%）。发现49个（78%）序列具有C-κB位点。ZA_LTR052被鉴定出有一个单点突变。我们在南非患者的病毒启动子-增强子区域中，在70%（44个）的样本中鉴定出了所有三个NF-κB结合位点。
