1 Biotechnology Department.
2 Institute of Food Engineering for Development, Food Technology Department.
J Food Prot. 2019 Mar;82(3):422-428. doi: 10.4315/0362-028X.JFP-18-355.
This study was conducted to assess the risk due to antimicrobial-resistant strains of Salmonella spp., Listeria monocytogenes, and Escherichia coli isolated from the eggshell and the contents of eggs bought in markets in Valencia (Spain). Thirty-four samples from three different production styles were analyzed: standard ( n = 34), organic ( n = 16), and backyard ( n = 10) eggs. L. monocytogenes was not isolated in any style of production. Only one strain of Salmonella was isolated from standard production, which was resistant to ciprofloxacin and amoxicillin. E. coli strains were resistant in 22% of the isolates from organic production, 12.25% from standard production, and 11.23% from backyard production. In all cases, the highest resistance was observed for amoxicillin-clavulanate. None of the isolates from standard and backyard eggs were resistant to chloramphenicol, ciprofloxacin, gentamycin, and streptomycin, while only ceftriaxone was found to be effective against all E. coli isolates from organic eggs. β-Lactamase genes bla , bla, and bla and the resistance genes for tetracycline tetA, tetB, and tetC were tested. The most commonly detected antimicrobial resistance genes among the E. coli isolates were tetA (49.30%), bla (47.89%), and tetB (36.62%). Overall, a maximum public health risk is associated with β-lactam antibiotics.
本研究旨在评估从瓦伦西亚（西班牙）市场购买的鸡蛋壳和内容物中分离出的沙门氏菌、单增李斯特菌和大肠杆菌的抗微生物耐药菌株的风险。分析了三种不同生产方式的 34 个样本：标准（n=34）、有机（n=16）和后院（n=10）鸡蛋。在任何生产方式中均未分离出单增李斯特菌。仅从标准生产中分离出一株对环丙沙星和阿莫西林耐药的沙门氏菌。在有机生产的 22%的分离株、标准生产的 12.25%和后院生产的 11.23%的大肠杆菌分离株中存在耐药性。在所有情况下，对阿莫西林-克拉维酸的耐药性最高。标准和后院鸡蛋的分离株均对氯霉素、环丙沙星、庆大霉素和链霉素耐药，而仅头孢曲松对所有有机鸡蛋的大肠杆菌分离株均有效。检测了β-内酰胺酶基因 blaCTX-M、blaTEM 和 blaOXA-1，以及四环素耐药基因 tetA、tetB 和 tetC。在大肠杆菌分离株中最常检测到的抗微生物耐药基因是 tetA（49.30%）、blaTEM（47.89%）和 tetB（36.62%）。总体而言，β-内酰胺类抗生素与最大的公共健康风险相关。
