Parker Deborah A, Goepfert J M
Food Research Institute, University of Wisconsin-Madison 1925 Willow Drive, Madison, Wisconsin 53706.
J Food Prot. 1978 Feb;41(2):116-117. doi: 10.4315/0362-028X-41.2.116.
Synthesis of Bacillus cereus enterotoxin was enchanced when the organism was grown by the sac-culture method. Of the three other major extracellular proteins examined, only lecithinase synthesis was enhanced. Advantages of the sac-culture method are that it helps simplify purification of B. cereus enterotoxin, and that while being simpler and more reduced in scale than that of a fermenter culture, it still yields the same enterotoxin levels on a per ml basis.