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乳制品中嗜酸乳杆菌可变菌株的计数

Enumeration of Vjable Lactobacillus acidophilus Organisms in Dairy Products.

作者信息

Speck M L

机构信息

Department of Food Science, North Carolina State University, Raleigh, North Carolina 27607.

出版信息

J Food Prot. 1978 Feb;41(2):135-137. doi: 10.4315/0362-028X-41.2.135.

DOI:10.4315/0362-028X-41.2.135
PMID:30795185
Abstract

Pasteurized low fat milk to which is added Lactobacillus acidophilus has now become a product of major volume on the U.S. market. The product contains millions of L. acidophilus per ml and is considered to be in the general category of cultured milk products for application of regulatory monitoring. There is a growing interest in developing standards for allowable minimum numbers of L. acidophilus in such products at point of sale. This can be accomplished best by use of a medium selective for lactobacilli such as Lactobacillus Selection Agar (LBS); non-selective media, such as APT agar, can also be used to enumerate L. acidophilus . The latter would provide less assurance that the counts were due to lactobacilli. The non-selectivity of APT, however, usually results in higher counts of L. acidophilus . There is need for application of accurate monitoring of such products to ensure the numbers as well as the identity of the culture used as the milk supplement.

摘要

添加了嗜酸乳杆菌的巴氏杀菌低脂牛奶现已成为美国市场上的一种大量生产的产品。该产品每毫升含有数百万嗜酸乳杆菌,被视为用于监管监测的发酵乳制品类别。人们越来越有兴趣制定此类产品在销售点的嗜酸乳杆菌允许最低数量标准。这最好通过使用对乳酸菌有选择性的培养基,如乳酸菌选择琼脂(LBS)来实现;非选择性培养基,如APT琼脂,也可用于嗜酸乳杆菌的计数。使用后者无法充分保证计数结果是由乳酸菌引起的。然而,APT的非选择性通常会导致嗜酸乳杆菌的计数更高。需要对这类产品进行准确监测,以确保作为牛奶补充剂使用的培养物的数量以及特性。

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