伊利诺伊州加工南瓜中辣椒疫霉菌株的特征分析

Characterization of Phytophthora capsici Isolates from Processing Pumpkin in Illinois.

作者信息

Islam S Z, Babadoost M, Lambert K N, Ndeme A, Fouly H M

机构信息

Department of Crop Sciences, University of Illinois, Urbana 61801.

Department of Natural Resources and Environmental Sciences, University of Illinois, Urbana 61801.

出版信息

Plant Dis. 2005 Feb;89(2):191-197. doi: 10.1094/PD-89-0191.

DOI:10.1094/PD-89-0191

PMID:30795223

Abstract

This study was conducted to investigate pathogenic, morphologic, and genetic variations among Phytophthora capsici isolates from processing pumpkin (Cucurbita moschata) fields in Illinois. Random amplified polymorphic DNA (RAPD) markers were employed to assess genetic variation among 24 isolates of P. capsici from 10 individual fields at six locations. Unweighted mean pair group analysis clustered isolates into six groups. The genetic distances ranged from 0.03 to 0.45. Inoculation of pumpkin seedlings in the greenhouse revealed that the isolates belonged to six distinct genetic groups differing significantly (P = 0.05) in virulence. Isolates tested exhibited four growth patterns in culture: cottony, rosaceous, petaloid, and stellate. P. capsici isolates, including an ATCC isolate (ATCC-15427), with cottony growth pattern did not grow at 36°C. The mean oospore diameter of A1 mating type isolates was greater than that of A2 mating type isolates. Nine of 24 isolates tested produced chlamydospores in V8-CaCO liquid medium.

摘要

本研究旨在调查伊利诺伊州加工南瓜（南瓜属）田中的辣椒疫霉分离株之间的致病性、形态学和遗传变异。采用随机扩增多态性DNA（RAPD）标记评估了来自六个地点10个不同田块的24株辣椒疫霉分离株之间的遗传变异。非加权平均配对组分析将分离株聚类为六组。遗传距离范围为0.03至0.45。温室中对南瓜幼苗的接种表明，这些分离株属于六个不同的遗传组，其毒力差异显著（P = 0.05）。测试的分离株在培养中表现出四种生长模式：棉絮状、玫瑰状、花瓣状和星状。包括ATCC分离株（ATCC - 15427）在内的呈棉絮状生长模式的辣椒疫霉分离株在36°C下不生长。A1交配型分离株的卵孢子平均直径大于A2交配型分离株。在24株测试分离株中，有9株在V8 - CaCO液体培养基中产生厚垣孢子。