Herron C M, Mirkov T E, N Solís-Gracia N, Kahlke C J, Skaria M, da Graça J V
Department of Plant Pathology and Microbiology, Texas A&M University System Agricultural Experiment Station.
Texas A&M University-Kingsville Citrus Center, Weslaco 78596.
Plant Dis. 2005 Jun;89(6):575-580. doi: 10.1094/PD-89-0575.
Citrus tristeza virus (CTV) isolates collected from the Lower Rio Grande Valley in south Texas and east Texas were characterized using citrus indicators and molecular methods. The citrus indicators were Mexican lime (Citrus aurantifolia), sour orange (C. aurantium), sweet orange (C. sinensis) grafted to sour orange, Duncan grapefruit (C. × paradisi), and Madam Vinous sweet orange, with some CTV isolates additionally indexed using the Texas commercial grapefruit cvs. Rio Red and Star Ruby, and Marrs and N-33 sweet orange. Severity ratings used 11 biotype groups or cumulative mean relative indices. Molecular characterization was carried out using poly- and monoclonal antibodies, seven strain-specific probes and single-stranded conformational polymorphism, and all were based on the CTV major coat protein or gene. All Texas CTV isolates produced vein clearing symptoms on inoculated Mexican lime plants. Over half of the CTV isolates tested were placed in biotype groups IX and X (causing decline of sweet orange on sour orange, seedling yellows on sour orange and grapefruit seedlings, and stem pitting of grapefruit or sweet orange), and one isolate was in biotype I (mild).
利用柑橘指示植物和分子方法对从得克萨斯州南部下里奥格兰德河谷和得克萨斯州东部采集的柑橘衰退病毒（CTV）分离株进行了鉴定。指示植物包括墨西哥莱檬（Citrus aurantifolia）、酸橙（C. aurantium）、嫁接到酸橙上的甜橙（C. sinensis）、邓肯葡萄柚（C. × paradisi）和维努斯夫人甜橙，部分CTV分离株还使用得克萨斯州商业葡萄柚品种里奥红和星红宝石以及马尔斯和N - 33甜橙进行了索引。严重程度评级采用11种生物型组或累积平均相对指数。分子鉴定使用了多克隆和单克隆抗体、7种菌株特异性探针和单链构象多态性，所有这些均基于CTV主要外壳蛋白或基因。所有得克萨斯州的CTV分离株在接种的墨西哥莱檬植株上均产生了脉明症状。超过一半的测试CTV分离株属于生物型组IX和X（导致甜橙在酸橙上衰退、酸橙和葡萄柚幼苗出现苗黄以及葡萄柚或甜橙出现茎陷点），还有一个分离株属于生物型I（轻度）。
